Inhibitory effects of carotenoids on the invasion of rat ascites hepatoma cells in culture
Inhibitory effects of carotenoids on the invasion of rat ascites hepatoma cells in culture
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DOI：
10.1016/s0304-3835(99)00418-8
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发表时间：
2000-04-03
期刊：
影响因子：
9.7
通讯作者：
Yagasaki, K
中科院分区：
文献类型：
--
作者：
Kozuki, Y;Miura, Y;Yagasaki, K
通过将肝癌细胞与大鼠肠系膜源间皮细胞（m细胞）共培养，研究了类胡萝卜素- -胡萝卜素、-胡萝卜素、番茄红素、-隐黄质、玉米黄质、叶黄素、角黄素、虾青素对大鼠腹水肝癌AH109A细胞侵袭的影响。所有的类胡萝卜素都以剂量依赖的方式抑制AH109A的侵袭，其影响可达5 μ M.用次黄嘌呤（HX）和黄嘌呤氧化酶（XO）培养的癌细胞显示出高度的侵袭活性。类胡萝卜素，5 μ M的β -胡萝卜素和虾青素，通过同时用类胡萝卜素，HX和XO处理AH109A细胞，抑制了这种活性氧增强的侵袭能力。这些结果表明，这些类胡萝卜素的抗氧化特性可能与它们的抗侵入作用有关。(C) 2000爱思唯尔科学爱尔兰有限公司版权所有。
The effects of carotenoids - alpha-carotene, beta-carotene, lycopene, beta-cryptoxanthin, zeaxanthin, lutein, canthaxanthin, astaxanthin - on the invasion of rat ascites hepatoma AH109A cells were investigated by co-culturing the hepatoma cells with rat mesentery-derived mesothelial cells (M-cells). All the carotenoids examined inhibited AH109A invasion in a dose-dependent manner up to 5 mu M. Cancer cells previously cultured with hypoxanthine (HX) and xanthine oxidase (XO) showed a highly invasive activity. Carotenoids, 5 mu M of beta-carotene and astaxanthin, suppressed this reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with the carotenoids, HX and XO. These results suggest that the antioxidative property of these carotenoids may be involved in their anti-invasive action. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.