Preliminary X‐ray studies of the tetra‐heme cytochrome c 3 and the octa‐heme cytochrome c 3 from Desulfovibrio gigas
Preliminary X‐ray studies of the tetra‐heme cytochrome c
3 and the octa‐heme cytochrome c
3 from Desulfovibrio gigas
复制标题
四血红素细胞色素 c 的初步 X 射线研究
DOI：
10.1016/0014-5793(86)81124-3
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
J. Legall
中科院分区：
文献类型：
--
作者：
L. Sieker;L. H. Jensen;J. Legall
从硫酸盐细菌Desulfovibrio gias中分离出一种四血红素和一种八血红素细胞色素3。在pH值为6.5的条件下加入聚乙二醇制得具有衍射性的四铁血红素细胞色素晶体。晶体属正交晶系，空间群为P21212，晶胞参数为sa=42.27，b=52.54，c=52.83。在pH 6.2-7.6的范围内，八氢血红素细胞色素晶体从低离子强度的磷酸盐或Tris-Cl溶液中生长出来。晶体属三方晶系，空间群P31或对映体P32，晶胞参数sa=b=57.4o，c=97.3o，γ=12 0°。对这两种低电势细胞色素结构的单晶衍射研究正在进行中。
A tetra‐heme and an octa‐heme cytochromec3from the sulfate bacteriumDesulfovibrio gigashave been crystallized. Diffraction quality crystals of the tetra‐heme cytochrome are obtained from solution by the addition of polyethylene glycol at pH 6.5. The crystals are orthorhombic, space group P21212 with unit cell parametersa= 42.27 Å,b= 52.54 Å andc= 52.83 Å. The octa‐heme cytochrome crystals develop from low ionic strength solutions of phosphate or Tris‐Cl in the pH range 6.2–7.6. The crystals belong to the trigonal system, space group P31or the enantiomorph P32, with unit cell parametersa=b= 57.4 Å,c= 97.3 Å, γ = 120°. Single crystal diffraction studies of the structures of these two low‐potential cytochromes are in progress.