Impact of migraine attacks on the blood-brain barrier
Impact of migraine attacks on the blood-brain barrier
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DOI：
10.3760/cma.j.issn.0366-6999.2010.18.012
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发表时间：
2010-09-20
影响因子：
6.1
通讯作者：
Zhang Zhong-ling
中科院分区：
文献类型：
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作者：
Gao Hong-mei;Li Le;Zhang Zhong-ling
背景皮质扩散性抑制可引起偏头痛发作，并上调基质金属蛋白酶9（MMP-9）的表达。本研究的目的是确定血脑屏障的结构和功能的影响，通过测量血浆MMP-9水平在偏头痛发作的急性和晚期患者，以阐明病理mechanisms involved.Methods我们招募了一个病例对照队列的38名成人偏头痛患者和20名年龄和性别匹配的健康对照组。在偏头痛的急性期和晚期（第1-7天）以及对照受试者中采集5毫升血液样本。采用固相双抗体夹心酶联免疫吸附法测定血浆MMP-9水平。结果偏头痛患者血浆MMP-9水平明显高于正常对照组（（12.612+/-0.016）μ g/L vs.（6.069 ± 0.023）μ g/L，P <0.05）;结论偏头痛可导致血浆MMP-9水平升高，从而导致血脑屏障损伤。MMP-9水平在偏头痛发作的第1-6天增加，在第3天达到峰值。因此，MMP-9可作为指导偏头痛发作治疗的生物学标志物。中华医学杂志2010;123（18）：2559-2561
Background Cortical spreading depression can cause migraine attack, and up-regulate matrix metalloproteinase-9 (MMP-9) expression in animal. This study aimed to determine the impact on the structure and function of the blood-brain barrier by measuring plasma MMP-9 levels in patients at the acute and late stages of migraine attacks in order to elucidate the pathological mechanisms involved.Methods We recruited a case-control cohort of 38 adult migraine patients and 20 age- and gender-matched healthy control subjects. Five milliliter blood samples were collected at the acute and late stages of migraine (days 1-7), and also from the control subjects. Solid phase double antibody sandwich enzyme-linked immunosorbent assay was used to determine plasma MMP-9 levels. Statistical analysis was performed using the SAS version 9.1.Results Initial plasma MMP-9 levels of migraine patients were significantly higher than those of controls ((12.612+/-0.016) mu g/L vs. (6.069 0.023) mu g/L, respectively, P 0.05); in addition, levels were not correlated with degree of headache pain (P >0.05).Conclusions We hypothesize that migraine could lead to increased plasma MMP-9 levels resulting in blood-brain barrier damage. MMP-9 levels increase during days 1-6 of migraine attacks, peaking on day 3. Therefore, MMP-9 could be used as a biological marker to guide treatment of migraine attacks. Chin Med J 2010;123(18):2559-2561