The importance of the spatial relationship of the gastric antrum in the development of gastrojejunal ulcer in the dog.
The importance of the spatial relationship of the gastric antrum in the development of gastrojejunal ulcer in the dog.
复制标题
胃窦的空间关系在狗胃空肠溃疡发展中的重要性。
DOI：
10.1007/s11033-004-0566-3
复制
发表时间：
1953
期刊：
影响因子：
--
通讯作者：
O. Wangensteen
中科院分区：
文献类型：
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作者：
W. D. Kelly;F. S. Cross;O. Wangensteen
我们分离的DNA片段含有各种重复元件从基因组的海鲷opagrus latus。序列分析表明，两个片段具有特别有趣的特征。片段AL 87含有一个四核苷酸重复序列和一个准回文序列。序列比较表明，AL 87可能是编码丝氨酸/苏氨酸蛋白激酶的基因的一部分，并且准回文序列位于内含子和外显子的连接处。此外，准回文序列在其他几种鱼类中是保守的，即使它有可能在剪接位点形成茎环结构。片段AL 79含有由6个30-bp单元串联组成的小卫星序列。DNA酶I敏感性测定和统计分析表明，重复区域是灵活的，当受到弯曲应力。此外，AL 79的原子力显微镜成像显示重复区域侧翼存在高度弯曲（扭结）的片段。这些重复元件的结构特征可能是促进重复序列扩增的关键因素。
We isolated DNA fragments containing various repetitive elements from the genome of a sea breamAcanthopagrus latus. Sequence analysis indicated that two fragments have particularly interesting features. Fragment AL87 contained a tetranucleotide repeat and a quasipalindromic sequence. Sequence comparison suggested that AL87 may be a part of a gene encoding a serine/threonine protein kinase, and that the quasipalindrome is situated at the junction of an intron and an exon. Moreover, the quasipalindrome is conserved in several other fishes, even though it has the potential to form a stem-loop structure at the splicing site. Fragment AL79 contained a minisatellite sequence made up of six 30-bp units in tandem. DNase I sensitivity assays and statistical analyses showed the repeat region to be flexible when subjected to bending stress. In addition, atomic force microscopic imaging of AL79 showed the presence of highly curved (kinked) segments flanking the repeat region. The structural features of these repetitive elements may be key factors facilitating the amplification of the repeats.