Collagen-binding domain of a Clostridium histolyticum collagenase exhibits a broad substrate spectrum both in vitro and in vivo
Collagen-binding domain of a Clostridium histolyticum collagenase exhibits a broad substrate spectrum both in vitro and in vivo
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DOI：
10.3109/03008200109016842
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发表时间：
2001-01-01
影响因子：
2.9
通讯作者：
Itano, T
中科院分区：
文献类型：
--
作者：
Toyoshima, T;Matsushita, O;Itano, T
研究了溶组织梭菌Ⅰ类胶原酶（ColG）串联胶原结合域（CBD）的体外和体内底物谱。CBD与不溶性I型结合。II、III和IV胶原蛋白，以及含有各种类型胶原原纤维或片的皮肤、主动脉、肌腱、肾、气管和角膜组织。CBD与各种胶原纤维结合，而不管它们的直径如何，并且还与肾小球基底层或角膜的后弹力膜中的成片胶原结合。这种宽的底物谱扩展了我们先前提出的药物递送系统的可能应用（PNAS 95：7018-7023，1998）。与CBD融合的治疗剂将不仅与皮下组织结合，而且与含有非I型胶原的其他组织结合。
The substrate spectrum of the tandem collagen-binding domain (CBD) of Clostridium histolyticumclass I collagenase (ColG) was examined both in vitro and in vivo. CBD bound to insoluble type I. II, III and IV collagens in vitro, and to skin, aorta, tendon, kidney, trachea and corneal tissues containing various types of collagen fibrils or sheets. CBD bound to all kinds of collagen fibrils regardless of their diameters and also bound to sheet-forming collagen in the glomerular basal lamina or Descemet's membrane of the cornea. This wide substrate spectrum expands possible applications of the drug delivery system we proposed previously (PNAS 95:7018-7023, 1998). Therapeutic agents fused with CBD will bind not only to subcutaneous tissues, but also to other tissues containing non-type I collagen.