CIRCADIAN-RHYTHM OF CELL-PROLIFERATION IN THE TELENCEPHALIC VENTRICULAR ZONE - EFFECT OF INUTERO EXPOSURE TO ETHANOL
CIRCADIAN-RHYTHM OF CELL-PROLIFERATION IN THE TELENCEPHALIC VENTRICULAR ZONE - EFFECT OF INUTERO EXPOSURE TO ETHANOL
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DOI：
10.1016/0006-8993(92)91447-m
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发表时间：
1992-11-06
期刊：
影响因子：
2.9
通讯作者：
MILLER, MW
中科院分区：
文献类型：
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作者：
MILLER, MW
新皮质脑室区是由一个去分化的增殖细胞群组成的。这些细胞在新皮层的颗粒下层产生神经元。本研究记录了心室区细胞增殖的昼夜节律性，并研究了乙醇对该生物钟的影响。妊娠大鼠饲喂3种饲料之一。在妊娠第6天至第18天期间，向其提供含乙醇（6.7% v/v）的液体饲料，自由进食，成对喂食等热量液体对照饲料，或喂食食物和水。在整个实验过程中，在08.00 h（E.S.T.）或在17.00 h（06.00至18.00 h开灯）。在24 h期间（03.00、06.00、09.00 h等）的一个时间点，在G17给大鼠单次注射溴脱氧尿苷（BrdU）。采用定量免疫组织化学方法测定掺入BrdU的心室细胞的分数。配对喂食的对照大鼠（在08.00或17.00 h喂食）在给药后4 h内进食。通过细胞周期S期的细胞比例每日变化;该比例在白天最高（12.00 h时为0.52 +/- 0.01），在夜间最低（03.00 h时为0.40 +/- 0.02）。相比之下，乙醇喂养的大鼠在整个黑暗周期中进食，无论食物何时出现。在黑暗阶段，平均峰值血液乙醇浓度为142 +/- 13 mg/dl，在光照阶段低于25 mg/dl。产前暴露于乙醇消除了胎儿细胞增殖的昼夜节律（平均标记指数为0.45 ± 0.03）。这种乙醇诱导的改变表明了发育系统的弹性;它能够补偿改变发育的表观遗传控制的创伤。
The neocortical ventricular zone is composed of a desynchronized population of proliferating cells. These cells give rise to neurons in the infragranular laminae of neocortex. The present study documents a diurnal rhythmicity of cell proliferation in the ventricular zone and examines the effects of ethanol on this biological clock. Pregnant rats were fed one of 3 diets. They were provided an ethanol-containing (6.7% v/v) liquid diet ad libitum between gestational day (G) 6 and G18, pair-fed an isocaloric liquid control diet, or fed chow and water. Throughout the experiments, the rats were fed either at 08.00 h (E.S.T.) or at 17.00 h (lights on 06.00 to 18.00 h). Rats were given a single injection of bromodeoxyuridine (BrdU) on G17 at one point during a 24 h period (03.00, 06.00, 09.00 h, etc.). The fraction of ventricular cells that incorporated the BrdU was determined using quantitative immunohistochemical methods. Pair-fed control rats (fed at 08.00 or 17.00 h) consumed their food within 4 h of presentation. The ratio of cells passing through the S-phase of the cell cycle changed diurnally; the ratio was highest during the day (0.52 +/- 0.01 at 12.00 h) and lowest during the night (0.40 +/- 0.02 at 03.00 h). In contrast, the ethanol-fed rats grazed on their food throughout the dark cycle regardless of when the food was presented. The mean peak blood ethanol concentration was 142 +/- 13 mg/dl during the dark phase and less than 25 mg/dl during the light phase. Prenatal exposure to ethanol eliminates the fetal circadian rhythm in cell proliferation (mean labeling index of 0.45 +/- 0.03). This ethanol-induced alteration demonstrates the resiliency of the developing system; its ability to compensate for traumas that alter the epigenetic control of development.