Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium
Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium
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DOI：
10.1016/j.bbrc.2008.05.185
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发表时间：
2008-08-22
影响因子：
3.1
通讯作者：
Mirshahi, Massoud
中科院分区：
文献类型：
--
作者：
Ducros, Elodie;Berthaut, Alexandre;Mirshahi, Massoud
在醛固酮作用8h的人骨髓内皮细胞(HBMEC)中，芯片筛选发现6个基因的mRNAs表达上调，4个基因的mRNAs表达下调，均与止血有关。在HBMEC中，免疫细胞化学显示膜结合型内皮蛋白C受体(EPCR)的存在，而盐皮质激素受体(MCR)则以核质形式存在。流式细胞仪分析和斑点印迹分析均显示，经醛固酮处理后的HBMEC可明显诱导EPCR蛋白的表达。当醛固酮处理的HBMEC与活化蛋白C共同孵育时，部分凝血活酶凝血时间(APTT)增加2.5倍，从10 S增加到25，MCR拮抗剂阿达克通和依普利酮使未处理细胞的基础凝血时间分别减少到对照组的33.5%和42%。这些数据增加了一个全新的维度来描述受体介导的矿物皮质激素的作用。(C)2008年，爱思唯尔公司出版。
In human bone marrow endothelial cell (HBMEC) exposed for 8 h to aldosterone, the microarray screening revealed an upregulation of the mRNAs for six genes and downregulation of mRNAs for four genes, all implicated in hemostasis. In HBMEC, immunocytochemistry revealed the presence of the membrane-bound endothelial protein C receptor (EPCR) whereas the mineralocorticoid receptor (MCR) was present as a nucleo-cytoplasmic. In HBMEC treated with aldosterone the induction of EPCR protein was evident by both FACS analysis and dot blot procedure. When aldosterone-treated HBMEC were incubated with the activated protein C (APC) the partial thromboplastin clotting time (aPTT) increased 2.5-fold over control, from 10 to 25 s. The MCR antagonists aldactone and eplerenone reduced the basal coagulation time in untreated cells to 33.5% and 42% of the control, respectively. These data add an entirely new dimension to delineating the receptor-mediated action of mineralocorticoid hormones. (C) 2008 Published by Elsevier Inc.