Directional axonal regrowth induced by an aligned fibrin nanofiber hydrogel contributes to improved motor function recovery in canine L2 spinal cord injury
Directional axonal regrowth induced by an aligned fibrin nanofiber hydrogel contributes to improved motor function recovery in canine L2 spinal cord injury
复制标题
对齐的纤维蛋白纳米纤维水凝胶诱导的定向轴突再生有助于改善犬 L2 脊髓损伤的运动功能恢复
DOI：
10.1007/s10856-020-06375-9
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发表时间：
2020-04-21
影响因子：
3.7
通讯作者：
Wang, Xiumei
中科院分区：
文献类型：
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作者：
Cao, Zheng;Yao, Shenglian;Wang, Xiumei
脊髓损伤（SCI）通常会破坏脊髓的长轴突束并导致永久性神经功能缺损，目前缺乏有效的治疗方法。基于生物材料的再生医学是通过生物材料传递生物物理和/或生化调节信号来诱导轴突再生的关键策略。我们之前制备了一种分层排列的纤维蛋白水凝胶（AFG），它可以在体外促进干细胞的神经原性分化，并已成功应用于大鼠周围神经和脊髓再生。本研究采用AFG修复犬腰椎2段半切脊髓损伤，并比较组织学、影像学和行为学结果的一致性。 AFG 用于构建对齐的纤维桥，在体外支持细胞粘附，并在体内快速促进沿纤维长轴的组织侵袭。此外，体内结果表明轴突以连接头端和尾端残端的定向模式重新生长。弥散张量成像证实了一致的结果，这使得能够成功追踪跨缺损处重新连接的神经纤维。结果，定向轴突再生有助于显着改善植入 AFG 的 SCI 犬运动功能行为的恢复。我们的结果表明 AFG 在快速引导轴突再生以实现神经再生方面具有巨大的前景。
Spinal cord injuries (SCI) normally disrupt the long axonal tracts of the spinal cord and cause permanent neurological deficits, for which there is currently a lack of effective therapeutic methods. Biomaterial-based regenerative medicine is a pivotal strategy to induce axonal regeneration through delivery of biophysical and/or biochemical regulatory cues by biomaterials. We previously fabricated a hierarchically aligned fibrin hydrogel (AFG) that could promote neurogenic differentiation of stem cells in vitro and has been successfully applied for peripheral nerve and spinal cord regeneration in rats. In this study, AFG was used to repair a canine lumbar segment 2 hemisection spinal cord injury, and the consistency of histological, imageological and behavioral results was compared. AFG was used to construct an aligned fiber bridge that supported cell adhesion in vitro and rapidly facilitated tissue invasion along the long axis of fibers in vivo, Moreover, in vivo results demonstrated regrowth of axons in an oriented pattern connecting the rostral and caudal stumps. Consistent results were confirmed by diffusion tensor imaging, which allowed successful tracing of reconnected nerve fibers across the defect. As a result, directional axonal regrowth contributed to significantly improved recovery of motor functional behavior of SCI canines with AFG implantation. Our results suggest that AFG has great promise for rapidly directing axonal regrowth for nerve regeneration.