Axonal motility and its modulation by activity are branch-type specific in the intact adult cerebellum
Axonal motility and its modulation by activity are branch-type specific in the intact adult cerebellum
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DOI：
10.1016/j.neuron.2007.09.010
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发表时间：
2007-11-08
期刊：
影响因子：
16.2
通讯作者：
Linden, David J.
中科院分区：
文献类型：
--
作者：
Nishiyama, Hiroshi;Fukaya, Masahiro;Linden, David J.
我们进行了双光子在体内成像的小脑攀爬纤维（CFs的橄榄小脑轴突的终端乔木）在成年小鼠。CF升支支配浦肯野细胞，而CIF横支显示几乎完全失败，形成传统的突触。几小时或几天的延时成像显示，升支非常稳定。然而，横支是高度动态的，表现出快速的伸长和收缩和静脉曲张周转。因此，同一轴突的不同分支，具有不同的神经支配模式，在成人小脑中显示分支类型特异性运动。此外，横向分支长度的动态变化几乎完全被药物刺激橄榄放电抑制。
We performed two-photon in vivo imaging of cerebellar climbing fibers (CFs; the terminal arbor of olivocerebellar axons) in adult mice. CF ascending branches innervate Purkinje cells while CIF transverse branches show a near complete failure to form conventional synapses. Time-lapse imaging over hours or days revealed that ascending branches were very stable. However, transverse branches were highly dynamic, exhibiting rapid elongation and retraction and varicosity turnover. Thus, different branches of the same axon, with different innervation patterns, display branch type-specific motility in the adult cerebellum. Furthermore, dynamic changes in transverse branch length were almost completely suppressed by pharmacological stimulation of olivary firing.