Trimethylation of histone H3K4 is associated with the induction of fructose-inducible genes in rat jejunum.
Trimethylation of histone H3K4 is associated with the induction of fructose-inducible genes in rat jejunum.
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DOI：
10.1016/j.bbrc.2012.02.043
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发表时间：
2012-03
影响因子：
3.1
通讯作者：
Y. Yoshinaga;K. Mochizuki;T. Goda
中科院分区：
文献类型：
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作者：
Y. Yoshinaga;K. Mochizuki;T. Goda
我们曾报道果糖强迫喂养可快速诱导大鼠空肠SLC2a5基因表达。在这项研究中，我们使用总RNA进行微阵列分析，以确定果糖强迫喂养下大鼠空肠中上调的基因。强制喂食果糖、葡萄糖或蒸馏水6h。与葡萄糖或蒸馏水相比，果糖胁迫可诱导SLc2a5、CDKN1c、Cabp2、Ranbp3、Vwce和GCGR等基因的表达。染色质免疫沉淀分析表明，强制喂食果糖可以促进组蛋白H3K4的三甲基化以及组蛋白H3和H4在这些果糖诱导基因转录区域的乙酰化，但不能促进葡萄糖或蒸馏水的作用。这些结果表明，果糖强迫喂养对大鼠空肠中基因的诱导是由组蛋白修饰，特别是组蛋白H3K4的三甲基化来协调调节的。
We previously reported that fructose force-feeding rapidly induces jejunal Slc2a5 gene expression in rats. In this study, we conducted microarray analyses using total RNA to identify genes upregulated in rat jejunum by fructose force-feeding. Rats were force-fed fructose, glucose or distilled water for 6h. Genes such as Slc2a5, Cdkn1c, Cabp2, Ranbp3, Vwce and Gcgr were induced by force-feeding with fructose compared with glucose or distilled water. Chromatin immunoprecipitation assays revealed that trimethylation of histone H3K4, and acetylation of histones H3 and H4, on the transcribed region of these fructose-inducible genes were enhanced by force-feeding of fructose, but not glucose or distilled water. These results suggest that the induction of genes in the rat jejunum by fructose force-feeding is coordinately regulated by histone modifications, particularly trimethylation of histone H3K4.