c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-Positive Bacillus thuringiensis.
c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-Positive Bacillus thuringiensis.
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c-di-GMP 调节革兰氏阳性苏云金芽孢杆菌的多种表型和杀虫活性
DOI：
10.3389/fmicb.2018.00045
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发表时间：
2018
影响因子：
5.2
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Fu Y;Yu Z;Liu S;Chen B;Zhu L;Li Z;Chou SH;He J
C-di-GMP在革兰氏阴性菌的生理学中起着重要作用。然而，其对革兰氏阳性菌的影响鲜为人知。为了进一步了解c-di-GMP在革兰氏阳性细菌中的功能，我们对已成功用作杀虫剂的革兰氏阳性昆虫病原菌苏云金芽孢杆菌（Bacillus thuringiensis）的c-di-GMP代谢酶及其生理功能进行了详细的研究。我们对B中10种可能的c-二GMP合成酶二鸟苷酸环化酶（DGCs）和c-二GMP降解酶磷酸二酯酶（PDE）进行了系统研究。苏云金芽孢杆菌BMB 171，并通过删除一个或多个pde基因人工提高BMB 171中的细胞内c-di-GMP水平。我们发现细胞内c-di-GMP水平的增加表现出与革兰氏阴性菌相似的活性，包括改变细胞运动性、生物膜形成和细胞-细胞聚集的活性。出乎意料的是，我们还发现了一种新的功能，表现为增加水平的c-di-GMP，以促进该细菌对棉铃虫的杀虫活性。通过全基因组转录组分析，我们发现4.3%的B。随着c-di-GMP水平的升高，苏云金杆菌基因的转录水平发生了变化，其中77.3%的基因产物参与了一些迄今为止在其他细菌中尚未报道的调控途径。总之，我们的研究代表了第一个全面的报告c-di-GMP代谢酶，其对表型的影响，和c-di-GMP介导的转录组在一个重要的革兰氏阳性菌。
C-di-GMP has been well investigated to play significant roles in the physiology of many Gram-negative bacteria. However, its effect on Gram-positive bacteria is less known. In order to more understand the c-di-GMP functions in Gram-positive bacteria, we have carried out a detailed study on the c-di-GMP-metabolizing enzymes and their physiological functions in Bacillus thuringiensis, a Gram-positive entomopathogenic bacterium that has been applied as an insecticide successfully. We performed a systematic study on the ten putative c-di-GMP-synthesizing enzyme diguanylate cyclases (DGCs) and c-di-GMP-degrading enzyme phosphodiesterases (PDEs) in B. thuringiensis BMB171, and artificially elevated the intracellular c-di-GMP level in BMB171 by deleting one or more pde genes. We found increasing level of intracellular c-di-GMP exhibits similar activities as those in Gram-negative bacteria, including altered activities in cell motility, biofilm formation, and cell-cell aggregation. Unexpectedly, we additionally found a novel function exhibited by the increasing level of c-di-GMP to promote the insecticidal activity of this bacterium against Helicoverpa armigera. Through whole-genome transcriptome profile analyses, we found that 4.3% of the B. thuringiensis genes were differentially transcribed when c-di-GMP level was increased, and 77.3% of such gene products are involved in some regulatory pathways not reported in other bacteria to date. In summary, our study represents the first comprehensive report on the c-di-GMP-metabolizing enzymes, their effects on phenotypes, and the transcriptome mediated by c-di-GMP in an important Gram-positive bacterium.