Increased transcription of CYP6D1 causes cytochrome P450 mediated insecticide resistance in house fly
Increased transcription of CYP6D1 causes cytochrome P450 mediated insecticide resistance in house fly
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DOI：
10.1016/s0965-1748(98)00039-3
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发表时间：
1998-08-01
影响因子：
3.8
通讯作者：
Scott, JG
中科院分区：
文献类型：
--
作者：
Liu, N;Scott, JG
杀虫剂抗药性是继续困扰动物和农作物害虫控制工作的一个主要问题。昆虫对杀虫剂产生抗性的一个重要机制是通过细胞色素 P450 微粒体单加氧酶（单加氧酶）介导的解毒作用增强。我们对这种抗性机制的认识中的一个根本差距是对昆虫如何表达高水平的特定细胞色素 P450 的理解。反抗。一。例如 P450、CYP6D1 会导致家蝇对拟除虫菊酯杀虫剂产生抗性，并且在学习拟除虫菊酯抗性 (LPR) 品系（与敏感品系相比）中表达水平（mRNA 和蛋白质）高出 9 倍。在用放线菌素 D 抑制转录后，测量了耐药菌株和敏感菌株中 CYP6D1 mRNA 的相对稳定性。在两种菌株中检测到 CYP6D1 mRNA 丰度下降的时间过程相同，表明 LPR 中 CYP6D1 的高水平表达并不是由于 mRNA 稳定性增加所致。使用体外连续转录测定来测量 CYP6D1 转录的相对速率。与易感菌株相比，LPR 菌株中产生的 CYP6D1 转录物的相对量高出 10 倍。这表明 CYP6D1 转录的增加是单加氧酶介导的杀虫剂抗性的根本原因。抗性菌株 (LPR) 中 CYP6D1 转录率的增加由两个因素控制：一个因素在常染色体 1 上，另一个因素在常染色体 2 上。(C) 1998 Elsevier Science Ltd。保留所有权利。
Insecticide resistance is a major problem that continues to plague efforts to control pests of animals and crops. An important mechanism by which insects become resistant to insecticides is via increased detoxification mediated by the cytochrome P450 microsomal monooxygenases (monooxygenases). One of the fundamental gaps in our knowledge about this resistance mechanism is an understanding of how insects express high levels of the specific cytochrome P450(s) responsible for. resistance. One. such P450, CYP6D1, causes resistance to pyrethroid insecticides in the house fly and is expressed at 9-fold higher levels (mRNA and protein) in the Learn Pyrethroid Resistant (LPR) strain (compared to susceptible strains). The relative stability of CYP6D1 mRNA in resistant and susceptible strains was measured following inhibition of transcription with actinomycin D. The same time course of decrease in CYP6D1 mRNA abundance was detected in both strains indicating that the high level of expression of CYP6D1 in LPR is not due to increased stability of the mRNA. The comparative rates of transcription of CYP6D1 were measured using an in vitro run-on transcription assay. The relative amount of CYP6D1 transcript produced in this assay was 10-fold greater in the LPR strain compared to the susceptible strain. This demonstrates that increased transcription of CYP6D1 is an underlying cause of monooxygenase-mediated insecticide resistance. The increased rate of transcription of CYP6D1 in the resistant strain (LPR) is controlled by two factors: one on autosome 1 and another on autosome 2. (C) 1998 Elsevier Science Ltd. All rights reserved.