Comparison of tunable bandpass reaction cell inductively coupled plasma mass spectrometry with conventional inductively coupled plasma mass spectrometry for the determination of heavy metals in whole blood and urine
Comparison of tunable bandpass reaction cell inductively coupled plasma mass spectrometry with conventional inductively coupled plasma mass spectrometry for the determination of heavy metals in whole blood and urine
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DOI：
10.1016/j.sab.2004.05.013
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发表时间：
2004-09
期刊：
影响因子：
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通讯作者：
D. E. Nixon;K. Neubauer;S. Eckdahl;J. Butz;M. Burritt
中科院分区：
文献类型：
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作者：
D. E. Nixon;K. Neubauer;S. Eckdahl;J. Butz;M. Burritt
评价了动态反应细胞™电感耦合等离子体质谱仪（DRC-ICP-MS）用于测定尿液和全血中的砷、铅、镉、汞和铊。评价了反应池条件对ArCl+和CaCl+多原子干扰的抑制。反应气体为氩气中的5%氢气。铅、镉、汞和铊在反应池排气的情况下测定。使用2.5%叔丁醇、0.5% HCl和2 mg Au/l加上Ga、Rh和Bi内标物的混合物稀释全血和尿液。使用加标至尿液基质中的酸性标准品水溶液进行校准。所有五种元素的尿液和全血添加校准曲线几乎相同。DRC-ICP-MS的检测限等于或优于传统ICP-MS。DRC-ICP-MS和传统ICP-MS的国家标准与技术研究所（NIST）SRM 2670和BioRad Lyphochek尿液金属质控品的运行内变异系数（CV）几乎相同。对于NIST 2670，DRC-ICP-MS中As、Pb、Cd和Hg的运行内CV分别为1.9%、4%、1.7%和1.7%，BioRad尿液的运行内CV分别为2.9%、1.8%、3.4%、1.7%和1.0%。BioRad Lyphochek全血质控品浓度和CV分别为：78 μg/l（3.8%）、284 μg/l（0.52%）和544 μg/l（0.9%）。除汞外，经认证的全血和尿液质控品在DRC-ICP-MS和常规ICP-MS上的日间CV均小于4%。
A Dynamic Reaction Cell™ inductively coupled plasma mass spectrometer (DRC-ICP-MS) was evaluated for the determination of arsenic, lead, cadmium, mercury, and thallium in urine and whole blood. Reaction cell conditions were evaluated for suppression of ArCl+and CaCl+polyatomic interferences. The reaction gas was 5% hydrogen in argon. Lead, cadmium, mercury, and thallium were determined with the reaction cell vented. Mixture of 2.5% t-butanol, 0.5% HCl, and 2 mg Au/l plus Ga, Rh, and Bi internal standards was used to dilute whole blood and urine. Calibration was achieved using aqueous acidic standards spiked into urine matrix. Urine and whole blood addition calibration curves were nearly identical for all five elements. DRC-ICP-MS detection limits were equivalent or better than conventional ICP-MS. Within run coefficients of variation (CV's) were nearly the same for DRC-ICP-MS and conventional ICP-MS for National Institute of Standards and Technology (NIST) SRM 2670 and BioRad Lyphochek Urine Metals Control. DRC-ICP-MS within run CV's for As, Pb, Cd, and Hg were 1.9%, 4%, 1.7%, and 1.7%, respectively, for NIST 2670 and 2.9%, 1.8%, 3.4%, 1.7%, and 1.0% for BioRad urine. BioRad Lyphochek Whole Blood control concentrations and CV's were: 78 μg/l (3.8%), 284 μg/l (0.52%), and 544 μg/l (0.9%). With the exception of mercury day-to-day CV's for certified whole blood and urine controls were less than 4% on both the DRC-ICP-MS and conventional ICP-MS.