Crystal Growth of Lysozyme Controlled by Laser Trapping
Crystal Growth of Lysozyme Controlled by Laser Trapping
复制标题
激光捕获控制溶菌酶晶体生长
DOI：
10.1021/cg401065h
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发表时间：
2014
影响因子：
3.8
通讯作者：
T. Sugiyama
中科院分区：
文献类型：
--
作者：
Jing;A. Miura;K. Yuyama;H. Masuhara;T. Sugiyama
我们展示了一个四晶的鸡蛋白溶菌酶（HEWL）在D2 O缓冲溶液中的生长控制的激光捕获与聚焦连续波（CW）近红外（NIR）激光束。焦斑位于距离自发产生的目标晶体边缘10 μm处，尽管焦斑尺寸远小于距离，但仍观察到晶体生长。用不同的激光功率和偏振态研究了四角晶系晶体（101）和{110}面的生长速率。在照射下观察到的速率与自发生长中的速率大不相同，即，{110}面的生长速率显示出取决于照射时间的大的降低或增加。这种不寻常的晶体生长行为的动力学和机制进行了讨论，从一个大的稳定域形成的HEWL类液团簇通过液体成核和生长的观点，并考虑到集群域的各向异性。
We demonstrate the growth of a tetragonal crystal of hen egg-white lysozyme (HEWL) in D2O buffer solution controlled by laser trapping with a focused continuous-wave (CW) near-infrared (NIR) laser beam. The focal spot was located at 10 μm away from the edge of the target crystal that was generated spontaneously, and the crystal growth was observed although the focal spot size was much smaller than the distance. The growth rate of (101) and {110} faces of the tetragonal crystal was examined with various laser powers and polarizations. The rate observed under the irradiation was much different from those in spontaneous growth, namely, the growth rate of the {110} face showed a large decrease or increase depending on the irradiation time. The dynamics and mechanism of this unusual crystal growth behavior is discussed from the viewpoint of a large stable domain formation of the HEWL liquidlike clusters through liquid nucleation and growth and by considering the anisotropy of the cluster domain.
影响因子：
3.8
作者：
Alexander, Andrew J.;Camp, Philip J.
通讯作者：
Camp, Philip J.
蛋白质结晶过程中簇成熟的成熟。
影响因子：
8.6
作者：
Streets AM;Quake SR
通讯作者：
Quake SR
影响因子：
15
作者：
Duffus, Carla;Camp, Philip J.;Alexander, Andrew J.
通讯作者：
Alexander, Andrew J.