Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
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DOI：
10.3791/51875
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发表时间：
2014-08-01
影响因子：
1.2
通讯作者：
Bettenworth, Dominik
中科院分区：
文献类型：
--
作者：
Brueckner, Markus;Lenz, Philipp;Bettenworth, Dominik
小鼠模型被广泛用于研究人类疾病的发病机制，并在临床前评估诊断程序以及治疗干预措施。然而，病理学改变的有效评估通常需要组织学分析，并且当离体进行时，需要动物死亡。因此，在传统的实验环境中，个体内随访检查很少可能。因此，在活小鼠中开发小鼠内窥镜检查使研究人员首次能够直接观察胃肠道粘膜，并重复该过程以监测变化。存在许多用于体内鼠内窥镜检查的应用，包括研究肠道炎症或伤口愈合，重复获得粘膜活检，以及使用微型注射导管局部施用诊断剂或治疗剂。最近，分子成像已经扩展了诊断成像模式，允许使用特异性光探针特异性检测不同的靶分子。总之，小鼠内窥镜已成为一种新的尖端技术，用于诊断实验体内成像，并可能对各个领域的临床前研究产生重大影响。
Mouse models are widely used to study pathogenesis of human diseases and to evaluate diagnostic procedures as well as therapeutic interventions preclinically. However, valid assessment of pathological alterations often requires histological analysis, and when performed ex vivo, necessitates death of the animal. Therefore in conventional experimental settings, intra-individual follow-up examinations are rarely possible. Thus, development of murine endoscopy in live mice enables investigators for the first time to both directly visualize the gastrointestinal mucosa and also repeat the procedure to monitor for alterations. Numerous applications for in vivo murine endoscopy exist, including studying intestinal inflammation or wound healing, obtaining mucosal biopsies repeatedly, and to locally administer diagnostic or therapeutic agents using miniature injection catheters. Most recently, molecular imaging has extended diagnostic imaging modalities allowing specific detection of distinct target molecules using specific photoprobes. In conclusion, murine endoscopy has emerged as a novel cutting-edge technology for diagnostic experimental in vivo imaging and may significantly impact on preclinical research in various fields.