CELL LINEAGE-SPECIFIC UNDERMETHYLATION OF MOUSE REPETITIVE DNA
CELL LINEAGE-SPECIFIC UNDERMETHYLATION OF MOUSE REPETITIVE DNA
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DOI：
10.1038/307284a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
ROSSANT, J
中科院分区：
文献类型：
--
作者：
CHAPMAN, V;FORRESTER, L;ROSSANT, J
在小鼠胚胎发生期间，建立了几个不同的细胞谱系。滋养外胚层和原始内胚层只产生胚外结构，而原始外胚层则形成胎儿特有的1-3。最近的研究表明，来自胚外组织的非活动X染色体的DNA修饰水平低于胚胎和成体组织。使用HpaII/MspI等裂体、Southern杂交和克隆探针，我们在这里表明，与原始外胚层衍生物相比，滋养外胚层和原始内胚层的所有衍生品的重复DNA序列都是低甲基化的。这与在成人体细胞组织中观察到的这些重复元素的高度甲基化状态形成对比。可能涉及特异性去甲基化或抑制新甲基化，或两种机制的组合。这些发现表明，依赖于DNA修饰的基因调控元素在胚外细胞谱系中可能是不同的。
Several distinct cell lineages are established during mouse embryogenesis. The trophectoderm and primitive endoderm give rise to extraembryonic structures alone, while the primitive ectoderm becomes the fetus proper1–3. Recent studies suggest that the levels of DNA modification are lower in inactive X chromosomes from extraembryonic tissues than in embryonic and adult somatic tissues. UsingHpaII/MspI isoschizomers, Southern blots and cloned probes, we show here that repetitive DNA sequences from all derivatives of the two extraembryonic lineages, trophectoderm and primitive endoderm, are substantially undermethylated compared with primitive ectoderm derivatives. This contrasts with the highly methylated state of these repetitive elements observed in adult somatic tissues. Specific demethylation or inhibition ofde novomethylation, or a combination of both mechanisms, may be involved. These findings suggest that elements of gene regulation dependent on DNA modification may be different in extraembryonic cell lineages.