Limitations of the Clausius-Mossotti function used in dielectrophoresis and electrical impedance studies of biomacromolecules
Limitations of the Clausius-Mossotti function used in dielectrophoresis and electrical impedance studies of biomacromolecules
复制标题
DOI：
10.1002/elps.201900057
复制
发表时间：
2019-09-01
期刊：
影响因子：
2.9
通讯作者：
Pethig, Ronald
中科院分区：
文献类型：
--
作者：
Pethig, Ronald
介电电泳（DEP）研究已经从细胞和细菌的微观尺度，通过病毒粒子的介观尺度到DNA和蛋白质的分子尺度。在所有这些研究的分析中，总是采用基于宏观静电学的克劳修斯-莫索蒂函数。这种做法的局限性进行了探讨，与结论是，它应该放弃DEP研究的蛋白质和修改为天然DNA。对于一般的大分子样品，结合分子尺度相互作用和永久偶极子的影响的DEP理论更合适。实验方法来测试这些结论提出。
Dielectrophoresis (DEP) studies have progressed from the microscopic scale of cells and bacteria, through the mesoscale of virions to the molecular scale of DNA and proteins. The Clausius-Mossotti function, based on macroscopic electrostatics, is invariably employed in the analyses of all these studies. The limitations of this practice are explored, with the conclusion that it should be abandoned for the DEP study of proteins and modified for native DNA. For macromolecular samples in general, a DEP theory that incorporates molecular-scale interactions and the influence of permanent dipoles is more appropriate. Experimental ways to test these conclusions are proposed.