Regional dura mater differentially regulates osteoblast gene expression
Regional dura mater differentially regulates osteoblast gene expression
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DOI：
10.1097/00001665-200305000-00015
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发表时间：
2003-05-01
影响因子：
0.9
通讯作者：
Longaker, MT
中科院分区：
文献类型：
--
作者：
Warren, SM;Greenwald, JA;Longaker, MT
最近的研究表明，区域分化的硬脑膜通过向覆盖缝合复合体中的成骨细胞提供生长因子来调节小鼠颅缝的命运。为了确定区域分化的硬脑膜是否能够影响成骨细胞基因表达的变化，建立了一种体外共培养体系，在该体系中，来自新生大鼠颅骨的成骨细胞培养物在无血清介质中生长，其中含有来自额后部(PF)或矢状面(SAG)硬膜组织的硬脑膜细胞，概括了硬脑膜下层与颅缝上覆的成骨细胞之间的原位关系。本研究通过分析与PF或SAG硬膜细胞共培养的成骨细胞的总RNA，研究了局部硬脑膜信号对成骨细胞基因表达的影响。检测细胞外基质分子(碱性磷酸酶、骨涎蛋白、骨桥蛋白和骨钙素)和转录因子MSX2的表达。与以前的数据一致，研究结果表明，与硬膜细胞共培养的成骨细胞经历了基因表达的变化，这表明成骨细胞分化程度更高。此外，数据表明，从PF缝线分离的区域性分化的硬脑膜比SAG缝线来源的硬膜细胞更能促进成骨基因的表达。这些数据支持缝线来源的硬脑膜细胞在体外的骨诱导作用，这可能对体内的缝合生物学有意义。
Recent studies have suggested that regionally differentiated dura mater regulates murine cranial suture fate by providing growth factors to the osteoblasts in the overlying suture complex. To determine if regionally differentiated dura mater is capable of effecting changes in osteoblast gene expression, an in vitro coculture system was established in which osteoblast-enriched cell cultures derived from neonatal rat calvaria were grown in serum-free media in the presence of dural cells derived from posterior frontal (PF) or sagittal (SAG) dural tissues, recapitulating the in situ relation between the underlying dura mater and the osteoblasts in the overlying cranial suture. In this study, the changes in osteoblast gene expression induced by signaling from regional dura mater were examined by analyzing total cellular RNA isolated from osteoblasts cocultured with PF or SAG dural cells. The expression of extracellular matrix molecules (alkaline phosphatase, bone sialoprotein, osteopontin, and osteocalcin) and the transcription factor Msx2 was assessed. Consistent with previous data, the findings demonstrate that osteoblasts cocultured with dural cells undergo changes in gene expression indicative of a more differentiated osteoblast. Additionally, the data suggest that regionally differentiated dura mater isolated from the PF suture enhances the expression of osteogenic genes to a greater extent than SAG suture-derived dural cells. These data support an osteoinductive role for suture-derived dural cells in vitro that may have implications for suture biology in vivo.