Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix
Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix
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免疫检查点在宫颈透明细胞癌中的表达及意义
DOI：
10.1155/2020/1283632
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发表时间：
2020-04-03
影响因子：
4.1
通讯作者：
Xiang, Yang
中科院分区：
文献类型：
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作者：
Zong, Liju;Zhang, Qianqian;Xiang, Yang
本研究的目的是研究免疫检查点蛋白、程序性细胞死亡配体1（PD-L1）、B7-H3、B7-H4和T细胞活化的V结构域IG抑制因子（VISTA）在宫颈透明细胞癌（CCC）（宫颈癌的一种罕见组织学亚型）中的表达水平及其意义。我们还比较了这些生物标志物在宫颈CCCs和宫颈鳞状细胞癌（SCC）中的表达状态。我们使用免疫组织化学染色评估了50例宫颈CCCs和100例SCC中PD-L1、B7-H3、B7-H4和VISTA的表达，并研究了这些标志物、临床病理特征和CCCs患者生存率之间的相关性。在检查的宫颈CCC样本中，PD-L1、B7-H3、B7-H4和VISTA分别为22%、16%、32%和34%。19个样本（38%）对所有4种标记物均为阴性，而31个样本（62%）表达至少1种标记物。这些标志物均与研究的临床病理变量或患者生存无关。PD-L1、B7-H3和VISTA在子宫颈SCC中的检出率显著高于CCC。我们的研究证实了免疫检查点蛋白在宫颈CCCs中的表达，并表明它们的非冗余和互补作用。因此，我们的数据表明，单克隆抗体免疫检查点阻断可能对宫颈CCC患者不够有效。
The purpose of this study was to investigate the expression levels of the immune checkpoint proteins, programmed cell death-ligand 1 (PD-L1), B7-H3, B7-H4, and V-domain Ig suppressor of T cell activation (VISTA), as well as the significance thereof, in clear cell carcinoma (CCC) of the cervix (a rare histological subtype of cervical cancer). We also compared the expression statuses of these biomarkers in cervical CCCs with those in cervical squamous cell carcinomas (SCCs). We evaluated the expression of PD-L1, B7-H3, B7-H4, and VISTA in 50 cervical CCCs and 100 SCCs using immunohistochemical staining and investigated the associations between these markers, clinicopathologic features, and survival in patients with CCCs. Of the cervical CCC samples examined, 22%, 16%, 32%, and 34% were positive for PD-L1, B7-H3, B7-H4, and VISTA, respectively. Nineteen samples (38%) were negative for all 4 of these markers, whereas 31 (62%) expressed at least 1 marker. None of these markers was associated with the investigated clinicopathologic variables or patient survival. PD-L1, B7-H3, and VISTA were observed significantly more frequently in SCCs than in CCCs of the cervix. Our study confirmed the expression of immune checkpoint proteins in cervical CCCs and indicated their nonredundant and complementary roles. As such, our data suggest that monotherapeutic immune checkpoint blockade may not be sufficiently effective in patients with cervical CCC.