Carbohydrate, organic Acid, and amino Acid composition of bacteroids and cytosol from soybean nodules.
Carbohydrate, organic Acid, and amino Acid composition of bacteroids and cytosol from soybean nodules.
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DOI：
10.1104/pp.85.3.768
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发表时间：
1987-11
期刊：
影响因子：
7.4
通讯作者：
J. Streeter
中科院分区：
文献类型：
--
作者：
J. Streeter
大豆根瘤菌（Bradyrhizobium japonicum）类菌体和大豆（Glycine max（L.）Merr.使用分离技术分析来自根瘤的胞质溶胶，该分离技术使得可以估计和校正在拟杆菌分离期间可能发生的浓度变化。类杆菌和胞质溶胶提取物进行分馏离子交换柱，并进行了分析，碳水化合物的组成，使用气液色谱法和有机酸和氨基酸的组成，使用高效液相色谱法。首次报道了以苯甲酰甲基衍生物形式分析植物组织中的有机酸，并揭示了根瘤中存在的几种未知有机酸。分离类杆菌和胞质溶胶所需的时间是变化的，并且通过计算浓度对时间的回归来估计在两个级分的分离期间单个化合物的浓度的显著变化。当发现具有统计学显著性的斜率时，通过将回归线外推至时间零点来估计真实浓度。在78个浓度估计值中，只有5种代谢产物在样品制备过程中浓度发生了统计学显著（5%水平）变化：细胞质中的葡萄糖、蔗糖和琥珀酸盐，以及拟杆菌中的d-松醇和丝氨酸。在质量基础上，类杆菌中的主要化合物是（浓度降序）：肌醇，d-手性肌醇，α，α-海藻糖，蔗糖，天冬氨酸，谷氨酸，d-松醇，精氨酸，丙二酸和葡萄糖。在比例基础上（类杆菌中的浓度占类杆菌+胞质溶胶组分中浓度的百分比），主要化合物为：α-氨基己二酸（94）、海藻糖（66）、赖氨酸（58）和精氨酸（46）。结果表明，代谢产物的浓度可以可靠地确定类杆菌。
Metabolites in Bradyrhizobium japonicum bacteroids and in Glycine max (L.) Merr. cytosol from root nodules were analyzed using an isolation technique which makes it possible to estimate and correct for changes in concentration which may occur during bacteroid isolation. Bacteroid and cytosol extracts were fractionated on ion-exchange columns and were analyzed for carbohydrate composition using gas-liquid chromatography and for organic acid and amino acid composition using high performance liquid chromatography. Analysis of organic acids in plant tissues as the phenacyl derivatives is reported for the first time and this approach revealed the presence of several unknown organic acids in nodules. The time required for separation of bacteroids and cytosol was varied, and significant change in concentration of individual compounds during the separation of the two fractions was estimated by calculating the regression of concentration on time. When a statistically significant slope was found, the true concentration was estimated by extrapolating the regression line to time zero. Of 78 concentration estimates made, there was a statistically significant (5% level) change in concentration during sample preparation for only five metabolites: glucose, sucrose, and succinate in the cytosol and d-pinitol and serine in bacteroids. On a mass basis, the major compounds in bacteroids were (descending order of concentration): myo-inositol, d-chiro-inositol, alpha,alpha-trehalose, sucrose, aspartate, glutamate, d-pinitol, arginine, malonate, and glucose. On a proportional basis (concentration in bacteroid as percent of concentration in bacteroid + cytosol fractions), the major compounds were: alpha-aminoadipate (94), trehalose (66), lysine (58), and arginine (46). The results indicate that metabolite concentrations in bacteroids can be reliably determined.