The receptor tyrosine phosphatase Dlar and integrins organize actin filaments in the Drosophila follicular epithelium
The receptor tyrosine phosphatase Dlar and integrins organize actin filaments in the Drosophila follicular epithelium
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DOI：
10.1016/s0960-9822(01)00420-1
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发表时间：
2001-09-04
期刊：
影响因子：
9.2
通讯作者：
Van Vactor, D
中科院分区：
文献类型：
--
作者：
Bateman, J;Reddy, RS;Van Vactor, D
背景：肌动蛋白结构的调节在许多组织中有助于维持适当的细胞结构。在果蝇卵巢的滤泡上皮中，围绕卵母细胞的细胞的基底表面上有一个肌动蛋白丝系统。这些丝已被假定为调节卵母细胞的伸长，但是，控制这种细胞骨架阵列的分子组成部分尚未understood.Results：我们发现，受体酪氨酸磷酸酶（RPTP）Dlar和整合素参与组织卵泡细胞中的基底肌动蛋白丝。Dlar和常见的β-整联蛋白亚基mys的突变导致卵母细胞伸长失败，这与适当的肌动蛋白丝组织的损失相关。免疫定位显示，在卵子发生的早期，Dlar被极化到膜上，其中细丝终止，但在发育后期变得通常分布，此时β-整联蛋白和Enabled特异性地与肌动蛋白细丝末端结合。救援实验指出，早期的极性Dlar定位为关键的功能。此外，克隆分析表明，Dlar或mys的损失影响肌动蛋白丝的极性在野生型细胞周围的突变组织，这表明相邻细胞之间的通信调节细胞骨架组织。最后，我们发现，两个整合素A亚基编码的新的，如果需要适当的卵母细胞伸长，这意味着多个组件的ECM是有益的协调肌动蛋白纤维polarity.Conclusions：Dlar合作与整合素协调肌动蛋白丝在基底表面的滤泡上皮。据我们所知，这是第一次直接证明RPTP对肌动蛋白细胞骨架的影响。
Background: Regulation of actin structures is instrumental in maintaining proper cytoarchitecture in many tissues. In the follicular epithelium of Drosophila ovaries, a system of actin filaments is coordinated across the basal surface of cells encircling the oocyte. These filaments have been postulated to regulate oocyte elongation; however, the molecular components that control this cytoskeletal array are not yet understood.Results: We find that the receptor tyrosine phosphatase (RPTP) Dlar and integrins are involved in organizing basal actin filaments in follicle cells. Mutations in Dlar and the common beta -integrin subunit mys cause a failure in oocyte elongation, which is correlated with a loss of proper actin filament organization. Immunolocalization shows that early in oogenesis Dlar is polarized to membranes where filaments terminate but becomes generally distributed late in development, at which time beta -integrin and Enabled specifically associate with actin filament terminals. Rescue experiments point to the early period of polar Dlar localization as critical for its function. Furthermore, clonal analysis shows that loss of Dlar or mys influences actin filament polarity in wild-type cells that surround mutant tissues, suggesting that communication between neighboring cells regulates cytoskeletal organization. Finally, we find that two integrin a subunits encoded by mew and if are required for proper oocyte elongation, implying that multiple components of the ECM are instructive in coordinating actin fiber polarity.Conclusions: Dlar cooperates with integrins to coordinate actin filaments at the basal surface of the follicular epithelium. To our knowledge, this is the first direct demonstration of an RPTP's influence on the actin cytoskeleton.