Nucleotide sequence of the genes encoding the major tail sheath and tail tube proteins of bacteriophage P2.
Nucleotide sequence of the genes encoding the major tail sheath and tail tube proteins of bacteriophage P2.
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DOI：
10.1016/0042-6822(91)90502-3
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发表时间：
1991-03
期刊：
影响因子：
3.7
通讯作者：
Larissa K. Temple;S. Forsburg;R. Calendar;Gail E. Christie;Gail E. Christie
中科院分区：
文献类型：
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作者：
Larissa K. Temple;S. Forsburg;R. Calendar;Gail E. Christie;Gail E. Christie
噬菌体P2可收缩尾部的主要结构成分是F1和F11蛋白，它们被认为分别是尾鞘和尾管蛋白。这两种蛋白质之前被映射到P2晚基因F1的基础上，在各种P2琥珀突变体的蛋白质合成模式。为了阐明基因排列，并提供与其他收缩性噬菌体尾部结构比较的基础，我们已经确定了编码这两种蛋白质的P2基因组区域的核苷酸序列。编码区的位置的theFam 4突变和N-末端氨基酸测序的两种蛋白质进行了确认。由每个编码区预测的分子量和氨基酸组成与实验确定的每种蛋白质的分子量和氨基酸组成完全一致。F11由一个新发现的P2晚期基因编码。这些蛋白质与它们在噬菌体T4中的功能同源物几乎没有相似之处。
The major structural components of the contractile tail of bacteriophage P2 are proteins F1and F11which are believed to be the tail sheath and tube proteins, respectively. Both proteins were mapped previously to the P2 late geneF1based on the pattern of protein synthesis in various P2 amber mutants. In order to clarify the gene arrangement and to provide a basis for structural comparisons with other contractile phage tails, we have determined the nucleotide sequence of the region of the P2 genome encoding these two proteins. The coding regions were confirmed by location of theFam4 mutation and by N-terminal amino acid sequencing of both proteins. The molecular weight and amino acid composition predicted by each of the coding regions correspond well to those determined experimentally for each protein. F11is encoded by a newly identified P2 late gene. These proteins bear little resemblance to their functional homologues in bacteriophage T4.