In vivo preservation of the hybrid layer by chlorhexidine
In vivo preservation of the hybrid layer by chlorhexidine
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DOI：
10.1177/154405910708600608
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发表时间：
2007-06-01
影响因子：
7.6
通讯作者：
Pashley, D.
中科院分区：
文献类型：
--
作者：
Carrilho, M. R. O.;Geraldeli, S.;Pashley, D.
据报道，宿主来源的蛋白酶降解未完全树脂过滤的牙本质的胶原基质。本研究验证了一种假设，即磷酸酸蚀后，在牙本质中应用基质金属蛋白酶抑制剂氯己定（CHX）可以防止或延迟树脂-牙本质结合的界面降解。在非龋性第三磨牙中的树脂粘结I类牙列的同侧对保持口内功能14个月。通过微拉伸强度测试和透射电镜观察评价树脂-牙本质粘结的保存情况。在体内粘结强度保持稳定的CHX处理标本，而粘结强度显着下降，在对照牙齿。CHX处理标本中树脂浸润的牙本质表现出正常的胶原网络结构完整性。相反，在对照样本中发现了纤维状网络的渐进性崩解。胶原基质的自动降解可发生在树脂渗透的牙本质中，但可通过应用合成蛋白酶抑制剂（如氯己定）来防止。
Host-derived proteases have been reported to degrade the collagen matrix of incompletely-resinin-filtrated dentin. This study tested the hypothesis that interfacial degradation of resin-dentin bonds may be prevented or delayed by the application of chlorhexidine (CHX), a matrix metalloproteinase inhibitor, to dentin after phosphoric acid-etching. Contralateral pairs of resin-bonded Class I restorations in non-carious third molars were kept under intra-oral function for 14 months. Preservation of resin-dentin bonds was assessed by microtensile bond strength tests and TEM examination. In vivo bond strength remained stable in the CHX-treated specimens, while bond strength decreased significantly in control teeth. Resin-infiltrated dentin in CHX-treated specimens exhibited normal structural integrity of the collagen network. Conversely, progressive disintegration of the fibrillar network was identified in control specimens. Auto-degradation of collagen matrices can occur in resin-infiltrated dentin, but may be prevented by the application of a synthetic protease inhibitor, such as chlorhexidine.