Different changes in the expression of multiple kinds of tight-junction proteins during ischemia-reperfusion injury of the rat ileum.
Different changes in the expression of multiple kinds of tight-junction proteins during ischemia-reperfusion injury of the rat ileum.
复制标题
大鼠回肠缺血再灌注损伤期间多种紧密结蛋白表达的不同变化。
DOI：
10.1267/ahc.05048
复制
发表时间：
2006-04-22
影响因子：
2.4
通讯作者：
Takamatsu, Tetsuro
中科院分区：
文献类型：
--
作者：
Inoue, Kaori;Oyamada, Masahito;Mitsufuji, Shoji;Okanoue, Takeshi;Takamatsu, Tetsuro
位于肠上皮最顶端的紧密连接（TJ）功能障碍被认为是导致肠道疾病的各种并发症的原因。然而，多种TJ蛋白在缺血再灌注损伤中的行为尚不清楚。为了确定在肠屏障恢复过程中TJ蛋白的表达和定位的变化，我们诱导大鼠肠缺血再灌注损伤，测量异硫氰酸荧光素-葡聚糖-4 kDa的粘膜-血液渗透性，并通过免疫共聚焦显微镜将其与ZO-1、occludin和claudin-1、-2、-3、-4和-5的时空变化进行比较。再灌注后1小时，绒毛被剥脱，屏障功能丧失。再灌注后6 ~ 24小时，绒毛上皮细胞迁移恢复，屏障功能以及ZO-1、occludin和claudin-1、-3和-5的网状表达恢复时间依赖性。相反，在缺血-再灌注损伤后，在未处理的对照中观察到的封闭蛋白-2和封闭蛋白-4的局部表达丧失，并被上皮细胞中从隐窝到绒毛的更广泛表达所取代，其中基底外侧封闭蛋白-4表达增加。这些数据表明，肠屏障功能的恢复与ZO-1、闭合蛋白和封闭蛋白-1、-3和-5的表达相关，而封闭蛋白-2和封闭蛋白-4显示出表达和定位的独特变化。
Dysfunction of tight junctions (TJs), located at the most apical part of the intestinal epithelium, is believed to result in various complications in intestinal disease. However, the behaviors of multiple kinds of TJ proteins during ischemia-reperfusion injury are not understood in detail. To determine changes in expression and localization of TJ proteins during intestinal-barrier recovery, we induced intestinal ischemia-reperfusion injury in rats, measured mucosa-to-blood permeability of fluorescein isothiocyanate-dextran-4 kDa, and compared it with spatiotemporal changes of ZO-1, occludin, and claudin-1, -2, -3, -4, and -5 by immunoconfocal microscopy. At 1 hour post-reperfusion, villi were denuded and intestinal-barrier function was lost. From 6 to 24 hours post-reperfusion, villous epithelium was restored by cell migration, and barrier function together with reticular pattern expression of ZO-1, occludin, and claudin-1, -3, and -5, recovered time-dependently. To the contrary, after ischemia-reperfusion injury, the localized expression of claudin-2 and claudin-4 observed in the non-treated control was lost and replaced with broader expression from crypts to villi with increased basolateral claudin-4 expression in epithelial cells. These data demonstrated that recovery of intestinal barrier function is associated with expression of ZO-1, occludin, and claudin-1, -3, and -5, whereas claudin-2 and claudin-4 show unique changes in expression and localization.
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灌注梭状芽胞杆菌肠毒素片段从紧密连接链中去除特定的克劳丁：直接参与Claudins在紧密连接屏障中的证据。
DOI：
10.1083/jcb.147.1.195
发表时间：
1999-10-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sonoda N;Furuse M;Sasaki H;Yonemura S;Katahira J;Horiguchi Y;Tsukita S
通讯作者：
Tsukita S
影响因子：
7.8
作者：
Furuse, Mikio;Hata, Masaki;Furuse, Kyoko;Yoshida, Yoko;Haratake, Akinori;Sugitani, Yoshinobu;Noda, Tetsuo;Kubo, Akiharu;Tsukita, Shoichiro
通讯作者：
Tsukita, Shoichiro
DOI：
10.1083/jcb.141.7.1539
发表时间：
1998-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Furuse M;Fujita K;Hiiragi T;Fujimoto K;Tsukita S
通讯作者：
Tsukita S
影响因子：
5.6
作者：
Escaffit, F;Boudreau, F;Beaulieu, JF
通讯作者：
Beaulieu, JF
DOI：
10.1152/ajpgi.00121.2002
发表时间：
2003-01-01
影响因子：
4.5
作者：
Little, D;Dean, RA;Blikslager, AT
通讯作者：
Blikslager, AT