MOLECULAR-CLONING OF 2 RECEPTORS FROM RAT-BRAIN WITH HIGH-AFFINITY FOR SALMON-CALCITONIN
MOLECULAR-CLONING OF 2 RECEPTORS FROM RAT-BRAIN WITH HIGH-AFFINITY FOR SALMON-CALCITONIN
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DOI：
10.1016/0014-5793(93)81078-e
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发表时间：
1993-07-05
期刊：
影响因子：
3.5
通讯作者：
BEAUMONT, K
中科院分区：
文献类型：
--
作者：
ALBRANDT, K;MULL, E;BEAUMONT, K
从大鼠脑背核区克隆了两个与鲑鱼降钙素具有高亲和力的受体。推导的cDNA克隆L2175-D20（命名为C1 a受体）的479个氨基酸的序列分别与人和猪降钙素受体的报道的序列有78%和66%的相同性。克隆U3237-A2编码一种受体（命名为C1 b），除了在第二个胞外结构域中插入一个37个氨基酸外，它与C1 a相同。COS-7细胞转染有与[I-125]鲑鱼降钙素高亲和力结合的转录本（对于C1 a，K（d）= 8 pM;对于C1 b，K（d）= 48 pM），并对鲑鱼降钙素产生反应，cAMP增加。组织分布研究表明，C1 a转录在大鼠脑，骨骼肌，肾脏和肺，而C1 b主要发现在脑。
Two receptors with high affinity for salmon calcitonin were cloned from the nucleus accumbens region of rat brain. The deduced 479 amino acid sequence of cDNA clone L2175-D20 (designated C1a receptor) is 78% and 66% identical with those reported for human and porcine calcitonin receptors, respectively. Clone U3237-A2 codes for a receptor (designated C1b) that is identical to C1a except fora 37 aminoacid insert in the second extracellular domain. COS-7 cells transfected with either transcript bound [I-125]salmon calcitonin with high affinity (K(d) = 8 pM for C1a; K(d) = 48 pM for C1b) and responded to salmon calcitonin with increases in cAMP. Tissue distribution studies revealed C1a transcript in rat brain, skeletal muscle, kidney and lung, whereas C1b was predominantly found in brain.