Toxicity of Silver Nanoparticles in Macrophages
Toxicity of Silver Nanoparticles in Macrophages
复制标题
DOI：
10.1002/smll.201202120
复制
发表时间：
2013-08-12
期刊：
影响因子：
13.3
通讯作者：
Sotiriou, Georgios A.
中科院分区：
文献类型：
--
作者：
Pratsinis, Anna;Hervella, Pablo;Sotiriou, Georgios A.
今天，银纳米颗粒(纳米银)广泛应用于纺织品、食品包装、家用设备和生物应用，促使人们更好地了解它们的毒性和生物相互作用。特别是，纳米银对哺乳动物细胞的细胞毒性尚不清楚，因为这种研究可能会受到纳米银涂层和缺乏颗粒大小控制的影响。在这里，使用火焰气溶胶技术，在惰性纳米结构二氧化硅上负载了定义明确的尺寸(5.7到20.4 nm)的纳米银。制备的纳米银对小鼠巨噬细胞的细胞毒性在体外进行了评估，因为这些细胞是哺乳动物摄取纳米银后第一批与之对抗的细胞之一。二氧化硅载体有利于制备的纳米银在生物悬浮液中的分散和稳定，并且不会应用其他可能干扰纳米银生物相互作用的涂层或官能化。通过X射线衍射和电子显微镜对纳米银颗粒进行了详细的表征，结果表明纳米银的尺寸得到了很好的控制。较小的纳米银粒子在水中分散时，以Ag+离子的形式释放或浸出其质量的较大部分。这强烈地影响了纳米银与小鼠巨噬细胞孵育时的细胞毒性。纳米银的大小决定了其细胞毒性的模式(银+离子特定和/或粒子特定)。小纳米银(10 Nm)的毒性。纳米银颗粒与巨噬细胞的直接相互作用对纳米银的毒性起主导作用。
Silver nanoparticles (nanosilver) are broadly used today in textiles, food packaging, household devices and bioapplications, prompting a better understanding of their toxicity and biological interactions. In particular, the cytotoxicity of nanosilver with respect to mammalian cells remains unclear, because such investigations can be biased by the nanosilver coatings and the lack of particle size control. Here, nanosilver of well-defined size (5.7 to 20.4 nm) supported on inert nanostructured silica is produced using flame aerosol technology. The cytotoxicity of the prepared nanosilver with respect to murine macrophages is assessed in vitro because these cells are among the first to confront nanosilver upon its intake by mammals. The silica support facilitates the dispersion and stabilization of the prepared nanosilver in biological suspensions, and no other coating or functionalization is applied that could interfere with the biointeractions of nanosilver. Detailed characterization of the particles by X-ray diffraction and electron microscopy reveals that the size of the nanosilver is well controlled. Smaller nanosilver particles release or leach larger fractions of their mass as Ag+ ions upon dispersion in water. This strongly influences the cytotoxicity of the nanosilver when incubated with murine macrophages. The size of the nanosilver dictates its mode of cytotoxicity (Ag+ ion-specific and/or particle-specific). The toxicity of small nanosilver (10 nm). Direct silver nanoparticle-macrophage interactions dominate the nanosilver toxicity at sizes larger than 10 nm.