Effect of hydrogen peroxide on Microcystic aeruginosa: Role of cytochromes P450
Effect of hydrogen peroxide on Microcystic aeruginosa: Role of cytochromes P450
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过氧化氢对铜绿微囊藻的影响：细胞色素 P450 的作用
DOI：
10.1016/j.scitotenv.2018.01.067
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发表时间：
2018-06-01
影响因子：
9.8
通讯作者：
Wen, Yuezhong
中科院分区：
文献类型：
--
作者：
Wang, Jia;Chen, Zunwei;Wen, Yuezhong
由于蓝藻水华对水质和生态健康的不利影响，已成为世界性的问题。为了解决这个问题，过氧化氢（H2O2）被认为是一种潜在的杀藻剂，因为处理后不会产生副产物，而且它可以选择性地杀死蓝藻。此外，有报道称细胞色素P450 （CYPs）与H2O2有关，但CYPs在蓝藻中调控H2O2的作用尚不清楚。本研究将CYPs自杀抑制剂1-氨基苯并三唑（ABT）添加到暴露于H2O2的代表性蓝藻微囊藻（M. aeruginosa）中。结果表明，cyp5介导H2O2对铜绿假单胞菌的影响。确切地说，与单独处理H2O2相比，ABT的添加对M. aeruginosa生长的抑制作用更大，活性氧水平也更高。同时，由于H2O2和ABT的共同作用，光合参数显著降低，胞外微囊藻毒素（MCs）含量增加，但MCs总量减少，ABT还增强了Fe的聚集，这可能是影响MCs光合作用和合成的原因。mcs合成基因（mcyA和mcyD）转录水平降低，mcs释放基因（mcyH）转录水平升高，光合基因（psaB、psbd1和rbcL）转录水平降低，证实了mcs合成基因（mcyA和mcyD）转录水平对mcs产生/释放和光合作用电子传递的影响。综上所述，本研究阐明了CYPs在H2O2对M. aeruginosa的不良反应中的中介作用，为H2O2作为潜在杀藻剂的解释提供了新的理论依据。(c) 2018 Elsevier B.V.版权所有
Cyanobacterial bloom has been rising as a worldwide issue owing to its adverse effects to water quality and ecological health. To solve this problem, hydrogen peroxide (H2O2) has been considered as a potential algaecide because no byproducts are generated after treatment and because it kills cyanobacteria selectively. In addition, cytochromes P450 (CYPs) was reported to be related with H2O2, but the roles of CYPs in the regulation of H2O2 in cyanobacteria have yet to be investigated. In this study, the CYPs suicide inhibitor 1-aminobenzotriazole (ABT) was added to the representative cyanobacteria Microcystis aeruginosa (M. aeruginosa) exposed to H2O2. The results showed that CYPsmediates the effects of H2O2 on M. aeruginosa. To be exact, the addition of ABT induced greater inhibitory effects on the growth and higher reactive oxygen species levels in M. aeruginosa comparing to those treated with H2O2 alone. At the same time, photosynthetic parameters significantly decreased, and the content of extracellular microcystins (MCs) increased but the total MCs decreased due to the combined effect of H2O2 and ABT. ABT also intensified the aggregation of Fe, which might explain the effects on photosynthesis and synthesis of MCs. Furthermore, the transcriptional levels of MCs-synthesis genes (mcyA and mcyD) decreased butMCs-release gene (mcyH) increased, and photosynthetic genes (psaB, psbD1and rbcL) decreased, which confirmed the effects on the MC production/release and electron transport of photosynthesis, respectively. In summary, this study illuminated the mediation role of CYPs in the adverse effects on M. aeruginosa induced by H2O2, thus providing new theoretical basis for the explanation of H2O2 as potential algaecide. (c) 2018 Elsevier B.V. All rights reserved.