Angiotensin II receptors of human and primate adrenal fasciculata and glomerulosa: correlations of binding and steroidogenesis.
Angiotensin II receptors of human and primate adrenal fasciculata and glomerulosa: correlations of binding and steroidogenesis.
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人类和灵长类动物肾上腺束状和肾小球的血管紧张素 II 受体：结合和类固醇生成的相关性。
DOI：
10.1016/0026-0495(84)90205-1
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
White,C
中科院分区：
文献类型：
--
作者：
Douglas,JG;Brown,GP;White,C
亚细胞部分和胶原酶分散分离的肾上腺束和肾小球细胞从人类和灵长类动物的肾上腺和皮质醇产生肿瘤已被用于研究血管紧张素II （Ang II）受体和类固醇的生物合成。肾小球的受体密度是束状体的3倍。良性分泌皮质醇的腺瘤与正常的束状肌没有区别，但恶性肿瘤的亲和力结合明显降低。Ang II的激动剂和拮抗剂类似物竞争与已知生物活性相称的结合位点。与束状组织结合的Kdof （2 nmol/L）与正常和腺瘤组织中皮质醇生物合成的ed50呈正相关。类似物结合在皮质醇的生物合成中也有相应的变化。在55 pmol/L时，肾小球细胞醛固酮生物合成的ed50显著降低。与肾小球相比，人类束状肌中的受体较少，对Ang II的反应较不敏感，这与之前在其他物种中的体外观察结果一致。这些研究表明，人类和灵长类动物肾上腺束状组织中可能存在一种与肾小球受体具有许多共同特征的生物学相关的Ang II受体。
Subcellular fraction and collagenase-dispersed isolated adrenal fasciculata and glomerulosa cells from human and primate adrenal glands and cortisol-producing tumors have been utilized to study angiotensin II (Ang II) receptors and steroid biosynthesis. The receptor density of glomerulosa was threefold higher than that of fasciculata. A benign cortisol-producing adenoma did not differ from normal fasciculata, but a malignant tumor had significantly lower affinity binding. Agonist and antagonist analogues of Ang II competed for binding sites commensurate with known biologic activity. The Kdof binding to fasciculata (2 nmol/L) correlated well with the ED50of cortisol biosynthesis in normal and adenomatous tissue. Analogue binding also had corresponding changes in cortisol biosynthesis. The ED50for aldosterone biosynthesis by glomerulosa cells was significantly lower at 55 pmol/L. Fewer receptors in human fasciculata as compared to glomerulosa and a less sensitive response to Ang II are consistent with previous in vitro observations in other species. These studies suggest that there may be a biologically relevant Ang II receptor of human and primate adrenal fasciculata that share many characteristics with the receptor of the glomerulosa.