Fatty Acid-Specific Alterations in Leptin, PPARα, and CPT-1 Gene Expression in the Rainbow Trout
Fatty Acid-Specific Alterations in Leptin, PPARα, and CPT-1 Gene Expression in the Rainbow Trout
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DOI：
10.1007/s11745-014-3939-y
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发表时间：
2014-10-01
期刊：
影响因子：
1.9
通讯作者：
Paolucci, Marina
中科院分区：
文献类型：
--
作者：
Coccia, Elena;Varricchio, Ettore;Paolucci, Marina
已知脂肪酸（FA）通过调节许多基因的表达来调节脂质代谢。为了更好地了解个体FA对虹鳟脂代谢相关基因的影响（虹鳟鱼），进行体外时程研究，其中12个单独的FA（丁酸4：0;辛酸8：0;棕榈酸（PAM）16：0;硬脂酸（STA）18：0;棕榈油酸16：1 n-7;油酸18：1 n-9; 11-顺式-二十碳烯酸20：1 n-9;亚油酸（LNA）18：2n-6; α-亚麻酸（ALA）18：3 n-3;二十碳五烯酸（EPA）20：5 n-3;二十二碳六烯酸（DHA）22：6 n-3;花生四烯酸（ARA）20：4 n-6）在虹鳟鱼肝切片中孵育。评价FA给药随时间推移对瘦素、PPARa和CPT-1（脂质氧化相关基因）表达的影响。Leptin mRNA的表达受饱和脂肪酸（SFA）和LNA的下调，受单不饱和脂肪酸（MUFA）和长链多不饱和脂肪酸（PUFA）的上调，而STA和ALA没有影响。SFA、MUFA、ALA、ARA和DHA可上调PPAR α和CPT-1 mRNA的表达，LNA和EPA可下调其表达。这些结果表明，有个别和特定的FA诱导的修改瘦素，过氧化物酶体增殖物激活受体α和CPT-1基因的表达在虹鳟鱼，它是设想，这样的结果可能会提供非常有价值的信息，为未来的实际应用在鱼类营养。
It is known that fatty acids (FA) regulate lipid metabolism by modulating the expression of numerous genes. In order to gain a better understanding of the effect of individual FA on lipid metabolism related genes in rainbow trout (Oncorhynchus mykiss), an in vitro time-course study was implemented where twelve individual FA (butyric 4:0; caprylic 8:0; palmitic (PAM) 16:0; stearic (STA) 18:0; palmitoleic16:1n-7; oleic 18:1n-9; 11-cis-eicosenoic 20:1n-9; linoleic (LNA) 18:2n-6; alpha-linolenic (ALA) 18:3n-3; eicosapentenoic (EPA) 20:5n-3; docosahexaenoic (DHA) 22:6n-3; arachidonic (ARA) 20:4n-6) were incubated in rainbow trout liver slices. The effect of FA administration over time was evaluated on the expression of leptin, PPAR alpha and CPT-1 (lipid oxidative related genes). Leptin mRNA expression was down regulated by saturated fatty acids (SFA) and LNA, and was up regulated by monounsaturated fatty acids (MUFA) and long chain PUFA, whilst STA and ALA had no effect. PPAR alpha and CPT-1mRNA expression were up regulated by SFA, MUFA, ALA, ARA and DHA; and down regulated by LNA and EPA. These results suggest that there are individual and specific FA induced modifications of leptin, PPAR alpha and CPT-1 gene expression in rainbow trout, and it is envisaged that such results may provide highly valuable information for future practical applications in fish nutrition.