Increased IL-6 and IL-8 levels in cervicovaginal secretions of patients with cervical cancer
Increased IL-6 and IL-8 levels in cervicovaginal secretions of patients with cervical cancer
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DOI：
10.1006/gyno.1999.5358
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发表时间：
1999-05-01
影响因子：
4.7
通讯作者：
Out, TA
中科院分区：
文献类型：
--
作者：
Tjiong, MY;van der Vange, N;Out, TA
目标。关于人乳头瘤病毒（HPV）永生化细胞系和几种宫颈癌细胞系产生IL-6的数据存在矛盾。然而，目前还没有关于宫颈洗液中细胞因子水平与宫颈瘤变之间关系的报道。本研究的目的是调查是否可以发现局部产生IL-6，以及这种细胞因子的水平是否与宫颈瘤变的严重程度有关。测量IL-8以获得可能起源于上皮的炎症细胞因子的额外信息。方法对35例浸润性宫颈癌患者、62例宫颈上皮内瘤变（CIN）患者和25例对照组进行宫颈阴道冲洗和血清检查。ELISA法检测IL-6、IL-8水平，采用HPV-16特异性PCR法和CPI/IIG PCR法检测宫颈涂片中HPV DNA，苏木精-伊红染色组织切片对炎症浸润进行组织学分析。在宫颈癌患者、GIN患者和对照组中，宫颈阴道洗液中IL-6的中位浓度为171 pg/ml（四分位数范围：54-780），22 pg/ml (
Objective. Conflicting data exist on IL-6 production by human papillomavirus (HPV) immortalized cell lines and several cervical carcinoma cell lines. However, no information has been reported on the levels of cytokines in cervicovaginal washings in relation to cervical neoplasia, The aim of this study was to investigate whether local production of IL-6 could be found and whether the level of this cytokine was related to the severity of cervical neoplasia. IL-8 was measured to obtain additional information on an inflammatory cytokine with possible epithelial origin.Methods, Cervicovaginal washings and sera were obtained from 35 patients with invasive cervical cancer, 62 patients with cervical intraepithelial neoplasia (CIN), and 25 control subjects. IL-6 and IL-8 levels were determined by ELISA, HPV DNA in cervical smears was detected by a HPV-16-specific PCR method and additionally by CPI/IIG PCR, Histological analysis of the inflammatory infiltrate was performed on hematoxylin-eosin-stained tissue sections.Results. In the patients with cervical cancer, those with GIN, and the controls, the median IL-6 concentration in cervicovaginal washings was 171 pg/ml (interquartile range: 54-780), 22 pg/ml (