Fatty acids in component of milk enhance the expression of the cAMP-response-element-binding-protein-binding protein (CBP)/p300 gene in developing rats
Fatty acids in component of milk enhance the expression of the cAMP-response-element-binding-protein-binding protein (CBP)/p300 gene in developing rats
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DOI：
10.1017/s0007114507831680
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Goda, Toshinao
中科院分区：
文献类型：
--
作者：
Mochizuki, Kazuki;Kawai, Hiromi;Goda, Toshinao
乳中的脂肪酸被认为在哺乳-断奶期间对大鼠小肠的成熟和基因表达起重要作用。在本研究中，我们确定了cAMP反应元件结合蛋白结合蛋白（CBP）/p300，这是染色质重塑因子之一，调节组蛋白乙酰化，在大鼠出生后的空肠mRNA水平。CBP/p300 mRNA在哺乳期和断奶中期（5 ~ 20日龄）表达水平较高，断奶末期和断奶后急剧下降。原位杂交结果还表明，断奶后，绒毛和基底膜中CBP/p300 mRNA水平明显下降。在17日龄的幼鼠，断奶到高脂肪饮食，表现出较高的水平CBp/p300 mRNA比那些断奶到低脂饮食。辛酸，油酸和亚油酸，这是牛奶中的主要脂肪酸成分，经口给药诱导空肠CBP/p300基因表达。本研究结果表明，牛奶成分中的脂肪酸增强小肠中CBP/p300基因的表达。
Fatty acids in milk are thought to play an important role in intestinal maturation and gene expression in the rat small intestine during the suckling-weaning period. In the present Study, we determined the jejunal mRNA level of the cAMP-response-element-binding-protein-binding protein (CBP)/p300, which is one of the chromatin remodelling factors and regulates histone acetylation, during the postnatal period in rats. The mRNA level of CBP/p300 was high during the suckling and middle of the weaning period (day 5 to 20) and then declined sharply to a low level at the end of the weaning period and after weaning. In situ hybridisation also showed that CBP/p300 mRNA levels in the villus as well as the basal membrane clearly decreased after weaning. Rat pups at age 17d, weaned to a high-fat diet, showed higher levels of CBp/p300 mRNA than those weaned to a low-fat diet. Oral administration of caprylic acid, oleic acid and linoleic acid, which are major fatty acid components in milk, induced jejunal CBP/p300 gene expression. The present results suggest that fatty acids in components of milk enhance expression of the CBP/p300 genes in the small intestine.