Effect of hepatic cytosol on postnatal drug metabolism.
Effect of hepatic cytosol on postnatal drug metabolism.
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肝细胞质对产后药物代谢的影响。
DOI：
10.1159/000457745
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Vidyasagar,D
中科院分区：
文献类型：
--
作者：
Evans,MA;Bhat,R;Papazafiratou,C;Vidyasagar,D
新鲜分离的兔肝细胞和 10,000 g 肝匀浆中吲哚美辛 O-去甲基化的出生后成熟与在兔肝微粒体中观察到的显着不同。添加出生后第10天兔子的肝细胞质（100,000 g上清液）可显着抑制成年兔肝微粒体对吲哚美辛O-去甲基化的影响。动力学研究表明，抑制是竞争性的，并且与胞质溶胶的浓度一致。肝微粒体药物代谢的出生后成熟模式可能部分与内源性胞质抑制剂浓度的变化有关。
Postnatal maturation for indomethacin O-demethylation in freshly isolated hepatocytes and 10,000 g liver homogenates of rabbit was found to be significantly different from that observed in rabbit hepatic microsomes. Addition of hepatic cytosol(100,000 g supernatant) from day 10 postnatal rabbits significantly inhibited indomethacin O-demethylation by hepatic microsomes from adult rabbits. Kinetic studies demonstrated that the inhibition was competitive and consistent with the concentration of cytosol. The pattern of postnatal maturation for hepatic microsomal drug metabolism may in part be related to changes in the concentration of endogenous cytosolic inhibitors.