FISHER INFORMATION FOR EMCCD IMAGING WITH APPLICATION TO SINGLE MOLECULE MICROSCOPY.
FISHER INFORMATION FOR EMCCD IMAGING WITH APPLICATION TO SINGLE MOLECULE MICROSCOPY.
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适用于单分子显微镜的 EMCCD 成像的 FISHER 信息。
DOI：
10.1109/acssc.2010.5757570
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ober,RaimundJ
中科院分区：
文献类型：
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作者：
Chao,Jerry;Ward,ESally;Ober,RaimundJ
电荷耦合器件(CCD)由于其高的量子效率，在单分子显微镜等生物应用中是一种重要的成像工具。然而，在极弱的光线条件下，电荷耦合器件通常是不合适的，因为它的读出噪声很容易淹没微弱的信号。取而代之的是，可以使用电子倍增电荷耦合器件(EMCCD)，其随机放大所采集的信号以消除读出噪声。我们以前已经提出了一个计算Fisher信息的框架，因此也就是Cramer-Rao下限，用于从光学显微镜产生的图像估计参数(例如，单分子位置)。在这里，我们发展了在这个框架内推导出与从EMCCD图像估计参数有关的性能测量所需的理论。我们的结果允许比较一个ccd和一个emccd的最佳精确度，可以用来估计它们所采集的图像的参数。
Owing to its high quantum efficiency, the charge-coupled device (CCD) is an important imaging tool employed in biological applications such as single molecule microscopy. Under extremely low light conditions, however, a CCD is generally unsuitable because its readout noise can easily overwhelm the weak signal. Instead, an electron-multiplying charge-coupled device (EMCCD), which stochastically amplifies the acquired signal to drown out the readout noise, can be used. We have previously proposed a framework for calculating the Fisher information, and hence the Cramer-Rao lower bound, for estimating parameters (e.g., single molecule location) from the images produced by an optical microscope. Here, we develop the theory that is needed for deriving, within this framework, performance measures pertaining to the estimation of parameters from an EMCCD image. Our results allow the comparison of a CCD and an EMCCD in terms of the best accuracy with which parameters can be estimated from their acquired images.