POLIOVIRUS MUTANTS RESISTANT TO NEUTRALIZATION WITH SOLUBLE CELL RECEPTORS
POLIOVIRUS MUTANTS RESISTANT TO NEUTRALIZATION WITH SOLUBLE CELL RECEPTORS
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DOI：
10.1126/science.2177226
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发表时间：
1990-12-14
期刊：
影响因子：
56.9
通讯作者：
RACANIELLO, VR
中科院分区：
文献类型：
--
作者：
KAPLAN, G;PETERS, D;RACANIELLO, VR
分离到了耐可溶性细胞受体中和的脊髓灰质炎病毒突变体。针对脊髓灰质炎病毒HeLa细胞受体的单抗阻断了可溶性受体抵抗(SRR)突变体的复制，表明突变体利用该受体进入细胞。SRR突变体与HeLa细胞和细胞膜的结合减少。然而，根据斑块大小和一步生长曲线判断，结合表型的减少对病毒复制没有重大影响。这些结果表明，使用可溶性受体作为抗病毒药物可能导致选择中和抗性突变体，这些突变体能够结合细胞表面受体，复制，并导致疾病。
Poliovirus mutants resistant to neutralization with soluble cellular receptor were isolated. Replication of soluble receptor-resistant (srr) mutants was blocked by a monoclonal antibody directed against the HeLa cell receptor for poliovirus, indicating that the mutants use this receptor to enter cells. The srr mutants showed reduced binding to HeLa cells and cell membranes. However, the reduced binding phenotype did not have a major impact on viral replication, as judged by plaque size and one-step growth curves. These results suggest that the use of soluble receptors as antiviral agents could lead to the selection of neutralization-resistant mutants that are able to bind cell surface receptors, replicate, and cause disease.