Sensory innervation of the guinea pig extraocular muscles: A 1,1′‐dioctadecyl‐3,3,3′3′‐tetramethylindocarbocyanine perchlorate tracing and calcitonin gene‐related peptide immunohistochemical study
Sensory innervation of the guinea pig extraocular muscles: A 1,1′‐dioctadecyl‐3,3,3′3′‐tetramethylindocarbocyanine perchlorate tracing and calcitonin gene‐related peptide immunohistochemical study
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DOI：
10.1002/(sici)1096-9861(19970331)380:1
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发表时间：
1997-03
影响因子：
2.5
通讯作者：
M. Aigner;J. Lukas;M. Denk;R. Mayr
中科院分区：
文献类型：
--
作者：
M. Aigner;J. Lukas;M. Denk;R. Mayr
由于不同物种在本体感受器方面存在巨大差异，因此眼外肌的感觉器官引起了人们的特别关注。采用荧光双标记方法研究了缺乏肌梭和腱器官的豚鼠眼外肌的感觉神经支配。通过死后将 1,1'-双十八烷基-3,3,3'3'-四甲基吲哚羰花青高氯酸盐 (Di-I) 应用于眼外肌神经来评估初级感觉核周。在三叉神经节的同侧眼部发现了追踪的神经元。这与其他物种的发现一致。通过免疫组织化学方法在三叉神经节内检测到降钙素基因相关肽（CGRP）。未观察到 CGRP 样免疫反应性周核体的体位组织。在来自眼外肌的初级传入周核细胞中发现了小（最大直径低于30μm）、中（最大直径在30至50μm之间）和大（最大直径大于50μm）三叉神经节细胞。在 CGRP 样免疫反应细胞中，仅观察到小型和中型细胞。双标记实验表明豚鼠眼外肌初级传入神经的 CGRP 含量。讨论了与神经节细胞以前形态类别的关系。具有 CGRP 样免疫反应性的初级传入神经元可能具有传出功能，也可能参与眼外肌的炎症过程。 J.Comp。内奥尔。 380:16–22，1997 年。© 1997 Wiley-Liss, Inc.
The sensory apparatus of the extraocular muscles attains special interest because of the great variation among different species with respect to the proprioceptors. The sensory innervation of the guinea pig extraocular muscles, lacking both muscle spindles and tendon organs, was investigated with a fluorescence double‐labelling method. Primary sensory perikarya were assessed by postmortem application of 1,1′‐dioctadecyl‐3,3,3′3′‐tetramethylindocarbocyanine perchlorate (Di‐I) to the extraocular muscle nerves. Traced neurons were found in the ipsilateral ophthalmic part of the trigeminal ganglion. This is in line with findings in other species. Calcitonin gene‐related peptide (CGRP) was detected immunohistochemically within the trigeminal ganglion. No somatotopic organization was observed for CGRP‐like immunoreactive perikarya. Small (maximal diameter below 30 μm), medium (maximal diameter between 30 and 50 μm), and large (maximal diameter larger than 50 μm) trigeminal ganglion cells were found among the primary afferent perikarya from extraocular muscles. Among CGRP‐like immunoreactive cells, only small and medium cells were observed. Double‐labelling experiments indicated the CGRP content of primary afferents of the guinea pig extraocular muscles. The relationship to former morphological categories of ganglion cells is discussed. Primary afferent neurons with CGRP‐like immunoreactivity might have efferent functions and might also be involved in inflammatory processes of extraocular muscles. J. Comp. Neuol. 380:16–22, 1997. © 1997 Wiley‐Liss, Inc.