Analysis of starch amylolysis using plots for first-order kinetics
Analysis of starch amylolysis using plots for first-order kinetics
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DOI：
10.1016/j.carbpol.2011.10.048
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发表时间：
2012-02-14
影响因子：
11.2
通讯作者：
Ellis, Peter R.
中科院分区：
文献类型：
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作者：
Butterworth, Peter J.;Warren, Frederick J.;Ellis, Peter R.
研究人员经常研究在体外与α-淀粉酶长时间孵育期间从淀粉中释放的产物。反应时间过程通常符合一阶速率方程的线性形式，即，In[（C-infinity-C-t）/C-infinity] = -kt。这个方程要求精确估计C-无穷大，即，反应结束时产物的浓度。从消化率曲线估计C无穷大可能是不可靠的。古根海姆方法不需要C-无穷大的先验知识，但似乎没有应用于淀粉水解数据。另一种方法也是可用的，其中将不同时间点的消化率曲线的斜率（LOS）的对数相对于时间作图。这允许估计的k和C-无穷大，也可以揭示是否发生变化的消化速度从快速到缓慢的消化进行。我们描述了古根海姆和LOS方法，并提供了他们的应用淀粉消化率数据的例子。(C)2011爱思唯尔有限公司保留所有权利。
Investigators often study product release from starches during prolonged incubations with alpha-amylase in vitro. The reaction time courses usually fit to a linear form of a first order rate equation, i.e., In[(C-infinity-C-t)/C-infinity] = -kt. This equation calls for an accurate estimate of C-infinity i.e., the concentration of product at the end of the reaction. Estimates of C-infinity from digestibility curves can be unreliable. The Guggenheim method does not require prior knowledge of C-infinity but seems not to have been applied to starch hydrolysis data. An alternative method is also available in which the logarithm of the slope (LOS) of a digestibility curve at various time points is plotted against time. This allows estimations of both k and C-infinity and can also reveal whether changes occur in digestion rate from rapid to slow as digestion proceeds. We describe the Guggenheim and LOS methods and provide examples of their application to starch digestibility data. (C) 2011 Elsevier Ltd. All rights reserved.