Flavonoids from tartary buckwheat induce G2/M cell cycle arrest and apoptosis in human hepatoma HepG2 cells
Flavonoids from tartary buckwheat induce G2/M cell cycle arrest and apoptosis in human hepatoma HepG2 cells
复制标题
苦荞中的黄酮类化合物可诱导人肝癌 HepG2 细胞的 G2/M 细胞周期停滞和细胞凋亡
DOI：
10.1093/abbs/gmu023
复制
发表时间：
2014-06-01
影响因子：
3.7
通讯作者：
Wang, Zhuanhua
中科院分区：
文献类型：
--
作者：
Li, Yuying;Duan, Shizhao;Wang, Zhuanhua
研究了苦荞籽和麸皮中三种黄酮类同质化合物槲皮素、异槲皮素和芦丁对人肝癌细胞系 HepG2 的细胞毒性和抗氧化活性。总抗氧化能力检测结果表明，槲皮素的抗氧化能力在生物反应系统中最强。 [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物]测定表明，槲皮素对 HepG2 细胞系表现出最强的细胞毒性作用。流式细胞术分析表明，槲皮素显着增加了活性氧的产生，并导致G(2)/M期阻滞，同时孵育48小时后细胞凋亡增加。槲皮素诱导的细胞凋亡涉及 p53 和 p21 上调，Cyclin D1、Cdk2 和 Cdk7 下调。这些结果表明槲皮素诱导G(2)/M阻滞、细胞凋亡和细胞死亡可能与p53和p21表达增加、Cyclin D1、Cdk2和Cdk7水平降低以及细胞中活性氧的产生有关。本研究将有助于更好地认识和充分利用植物黄酮类药用资源。
The cytotoxicity and antioxidant activity on human hepatoma cell line HepG2 of three flavonoids homogenous compounds from tartary buckwheat seeds and bran, namely quercetin, isoquercetin, and rutin, were investigated. The total antioxidant competency detection results indicated that the antioxidant capacity of quercetin was the strongest in a biological response system. A [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay showed that quercetin exhibited the strongest cytotoxic effects against the HepG2 cell line. Flow cytometric analysis indicated that quercetin significantly increased the production of reactive oxygen species, and led to the G(2)/M phase arrest accompanied by an increase of apoptotic cell death after 48 h of incubation. Quercetin-induced cell apoptosis was shown to involve p53 and p21 up-regulation, Cyclin D1, Cdk2, and Cdk7 down-regulation. These results suggested that the induction of G(2)/M arrest, apoptosis, and cell death by quercetin may associate with increased expression of p53 and p21, decrease of Cyclin D1, Cdk2, and Cdk7 levels, and generation of reactive oxygen species in cells. This study will help to better understand and fully utilize medicinal resources of plant flavonoids.