SURFACE DOMAINS OF THE GUINEA-PIG SPERM DEFINED WITH MONOCLONAL-ANTIBODIES
SURFACE DOMAINS OF THE GUINEA-PIG SPERM DEFINED WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0092-8674(81)90138-0
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
BELLVE, AR
中科院分区：
文献类型：
--
作者：
MYLES, DG;PRIMAKOFF, P;BELLVE, AR
制备了四种识别豚鼠精子表面分子的单克隆抗体。每个抗体都与精子表面的一个独特的局部区域结合。这4种抗体结合模式仅限于精子的前头、后头、整个头和后尾。根据吸收实验，抗体检测到的表面分子为分化抗原。三种抗体免疫沉淀125i标记的蛋白质抗原。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后的多肽分子量分别为52000（前头）、42000（全头）和60000（后头）。观察到的单克隆抗体结合模式表明，豚鼠精子表面至少分为4个结构域。
Four monoclonal antibodies were produced that recognize surface molecules of the guinea pig sperm. Each antibody binds to a unique, localized region of the sperm surface. The 4 antibody-binding patterns are restricted to the anterior head of the sperm, the posterior head, the whole head and the posterior tail. Based on absorption experiment, the surface molecules detected by the antibodies are differentiation antigens. Three of the antibodies immunoprecipitate 125I-labeled protein antigens. The MW of the polypeptides observed after sodium dodecyl sulfate-polyacrylamide gel electrophoresis are 52,000 (anterior head), 42,000 (whole head) and 60,000 (posterior head). The observed monoclonal antibody-binding patterns suggest that the guinea pig sperm surface is divided into a minimum of 4 domains.