Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein
Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein
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DOI：
10.1093/nar/gkl562
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发表时间：
2006-09-01
影响因子：
14.9
通讯作者：
Yokoyama, Shigeyuki
中科院分区：
文献类型：
--
作者：
Sarai, Naoyuki;Kagawa, Wataru;Yokoyama, Shigeyuki
同源重组过程对于减数分裂和有丝分裂都是必不可少的，也是双链断裂(DSB)修复的主要途径之一。人的Rad54B蛋白属于SWI2/SNF2蛋白家族，参与同源重组，可能与减数分裂特异的RAD51同源物Dmc1重组酶一起发挥作用。在本研究中，我们发现Rad54B通过稳定Dmc1-单链DNA(SsDNA)复合体来增强Dmc1的DNA链交换活性。因此，Rad54B可能通过稳定核蛋白细丝来刺激Dmc1介导的DNA链交换，核蛋白细丝形成于同源重组过程中在DSB位点产生的单链DNA尾巴上。
The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.