Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development
Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development
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DOI：
10.1242/bio.052928
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发表时间：
2020-05-01
期刊：
影响因子：
2.4
通讯作者：
Southall, Tony D.
中科院分区：
文献类型：
--
作者：
Estacio-Gomez, Alicia;Hassan, Amira;Southall, Tony D.
神经系统中神经元的显着多样性是在发育过程中产生的，当细胞形态，受体谱和神经递质身份等特性被指定时。为了获得更好的理解神经递质的规范，我们分析了在体内的三个发育时间点的胆碱能，γ-氨基丁酸能和谷氨酸能神经元的转录状态。我们确定了86个差异表达的转录因子，这些转录因子在特定的神经递质类型中独特地富集或独特地耗尽。一些转录因子在整个发育过程中表现出类似的特征，另一些转录因子仅在特定的发育阶段表现出富集或耗尽。Acj 6（胆碱能富集）和Ets 65 A（胆碱能耗尽）结合位点的体内分析揭示，除了广泛的其他关键神经元分化基因之外，它们都直接结合ChATIocus。我们还表明，胆碱能富集的转录因子表达在大多数非重叠的人口在成年人的大脑，这意味着在这种情况下的神经递质的命运组合调节的情况下。此外，我们的数据强调，类似秀丽隐杆线虫，有没有简单的转录因子代码的神经递质类型specification.This文章有相关的第一人称采访的第一作者的文件。
The remarkable diversity of neurons in the nervous system is generated during development, when properties such as cell morphology, receptor profiles and neurotransmitter identities are specified. In order to gain a greater understanding of neurotransmitter specification we profiled the transcription state of cholinergic, GABAergic and glutamatergic neurons in vivo at three developmental time points. We identified 86 differentially expressed transcription factors that are uniquely enriched, or uniquely depleted, in a specific neurotransmitter type. Some transcription factors show a similar profile across development, others only show enrichment or depletion at specific developmental stages. Profiling of Acj6 (cholinergic enriched) and Ets65A (cholinergic depleted) binding sites in vivo reveals that they both directly bind the ChATIocus, in addition to a wide spectrum of other key neuronal differentiation genes. We also show that cholinergic enriched transcription factors are expressed in mostly non-overlapping populations in the adult brain, implying the absence of combinatorial regulation of neurotransmitter fate in this context. Furthermore, our data underlines that, similar to Caenorhabditis elegans, there are no simple transcription factor codes for neurotransmitter type specification.This article has an associated First Person interview with the first author of the paper.