STUDY ON THE PATHOGENIC FACTORS OF KASHIN-BECK DISEASE
STUDY ON THE PATHOGENIC FACTORS OF KASHIN-BECK DISEASE
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DOI：
10.1080/15287399209531597
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发表时间：
1992-02-01
期刊：
影响因子：
--
通讯作者：
LI, H
中科院分区：
文献类型：
--
作者：
PENG, A;YANG, CL;LI, H
大骨节病是一种慢性骨关节炎性疾病，在我国部分地区流行。其病因尚不清楚。硒缺乏、饮用水中高浓度有机物（主要是富里酸）和粮食受真菌严重污染已被认为是环境原因。自由基是环境因素与大骨节病发病之间可能的媒介物。来自大骨节病疫区的饮用水中半醌自由基含量高于来自无病区的饮用水。在动物实验中，富里酸（FA）以半醌自由基的形式在骨骼系统中积累。枯萎病菌或链格孢菌污染的粮食显着增加半醌自由基的含量。此外，在流行区种植的玉米比来自无病区的玉米具有更高的自由基含量。污染玉米中这些自由基的g因子值约为2.0040，在半醌自由基的范围内。在单层培养的人胚软骨细胞中，FA和镰刀菌污染的谷物水提物损伤软骨细胞并增强脂质过氧化。亚硒酸盐和超氧化物歧化酶（SOD）保护细胞免受这些毒素的损伤，并减少脂质过氧化物。生活在KBD疫区的儿童谷胱甘肽过氧化物酶活性较低，脂质过氧化水平较高。因此，被视为外源性自由基载体的FA和真菌毒素是大骨节病发病机制中的重要环境因素;而抑制自由基形成的硒、维生素C和维生素E被认为具有保护作用。
Kashin-Beck disease (KBD) is a chronic osteoarthritic disease, endemic in parts of China. Its etiology is unknown. Selenium deficiency, high concentration of organic matter (mainly fulvic acid) in drinking water, and severe contamination of grain by fungi have been proposed environmental causes. Free radicals, possible mediators between the environmental factors and origin of KBD, have been studied in this work. Drinking water from KBD-affected areas contains a higher level of semiquinone radicals than that from disease-free areas. In animal experiments, fulvic acid (FA) accumulated in the skeletal system as semiquinone radicals. Contamination of grain by Fusarium oxysporum or Alternaria alternata significantly increased the content of semiquinone radicals. Furthermore, corn grown in endemic areas had a higher content of radicals than that from disease-free areas. The g factor values for these radicals from contaminated corn were about 2.0040, in the range of semiquinone radical. In monolayer culture of human embryonic chondrocytes, FA and aqueous extracts of grain contaminated by Fusarium injured the chondrocytes and enhanced lipid peroxidation. Selenite and superoxide dismutase (SOD) protected the cells from injury by these toxins and reduced lipid peroxide. Lower glutathione peroxidase activities and higher levels of lipid peroxidation were also found in the children living in KBD-affected regions. Thus, FA and the mycotoxin, which are seen as exogeneous free-radical carriers, are important environmental factors in the pathogenesis of Kashin-Beck disease; and selenium, vitamin C, and vitamin E, which inhibit free-radical formation, are considered to be protective.