Suppression of the immune response by alpha-fetoprotein 1. The effect of mouse alpha-fetoprotein on the primary and secondary antibody response.
Suppression of the immune response by alpha-fetoprotein 1. The effect of mouse alpha-fetoprotein on the primary and secondary antibody response.
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甲胎蛋白对免疫反应的抑制 1. 小鼠甲胎蛋白对一抗和二抗反应的影响。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
J. TomasiTb
中科院分区：
文献类型：
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作者：
Murgita Ra;J. TomasiTb
小鼠羊水（AF）含有一种对绵羊红细胞无细胞毒性的原发性免疫球蛋白（Ig） M和次级免疫球蛋白（IgM） IgA和IgG亚类脾斑块形成细胞（PFC）的抑制剂。穷尽透析或羊红细胞吸收小鼠心房纤颤均不能消除其抑制作用。聚丙烯酰胺凝胶分析显示，透析后的小鼠心房颤动主要由转铁蛋白、白蛋白和甲胎蛋白（AFP） 3种主要蛋白组分组成。亲和层析法从心房纤颤中选择性去除这些蛋白表明AFP是小鼠心房纤颤中的免疫抑制物质。这一结论得到了证实，证明纯AFP能抑制mcg量的体外抗体合成，而等量的正常小鼠血清转铁蛋白或白蛋白则没有。剂量-反应研究表明，分离形式的AFP的作用与相当量的AFP在小鼠AF中的抑制作用相当。与IgM PFC反应相比，AFP浓度明显较低，可抑制IgA和IgG PFC反应。抑制的程度取决于AFP加入培养的时间；在培养开始后24小时将小鼠AF添加到抗原刺激培养物中具有免疫抑制作用，而在开始后48小时或更晚添加类似的小鼠AF则没有抑制作用。在没有抗原的情况下，将脾细胞暴露于小鼠房颤培养液中，以达到抑制随后的原发性免疫反应的目的，持续时间为8小时。因此AFP可能具有免疫调节功能。
Mouse amniotic fluid (AF) was shown to contain a noncytotoxic inhibitor of primary immunoglobulin (Ig) M and secondary IgM IgA and IgG subclass splenic plaque forming cells (PFC) in vitro to sheep red blood cells. The suppressive effect was not abolished by exhaustive dialysis or by absorption of mouse AF with sheep erythrocytes. Polyacrylamide gel analysis showed that dialyzed mouse AF was composed of 3 major protein components transferrin albumin and alpha fetoprotein (AFP). Selective removal of each of these proteins from AF by affinity chromatography suggested that AFP was the immunosuppressive substance in mouse AF. This conclusion was verified by the demonstration that pure AFP suppressed in vitro antibody synthesis in mcg quantities whereas equivalent amounts of normal mouse serum transferrin or albumin did not. Dose-response studies showed that the effect of AFP in the isolated form was equivalent to the suppressive effect of comparable amounts of AFP in mouse AF. IgA and IgG PFC responses were suppressed by a significantly lower concentration of AFP than was the IgM PFC response. The degree of suppression was dependent on the time at which AFP was added to the cultures; mouse AF added to antigen-stimulated cultures up to 24 hours after initiation of cultures was immunosuppressive whereas similar additions of mouse AF at 48 hours after initiation or later did not suppress. Duration of exposure of spleen cells to mouse AF in cultures without antigen necessary to achieve suppression of a subsequent primary immune response was determined to be 8 hours. AFP therefore may have an immunoregulatory function.