Enzymatic hydrolysis and ethanol fermentation of high dry matter wet-exploded wheat straw at low enzyme loading
Enzymatic hydrolysis and ethanol fermentation of high dry matter wet-exploded wheat straw at low enzyme loading
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DOI：
10.1007/s12010-007-8085-z
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发表时间：
2008-03-01
影响因子：
3
通讯作者：
Ahring, Birgitte K.
中科院分区：
文献类型：
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作者：
Georgieva, Tania I.;Hou, Xiaoru;Ahring, Birgitte K.
采用三种不同的氧化剂（H2 O2、O-2和空气）对小麦秸秆进行湿爆预处理。基于酶水解过程中释放的葡萄糖和木糖来评价预处理的效果。结果表明，使用O-2作为氧化剂的预处理在提高原料向糖的总体转化率和最小化作为副产物的糠醛的生成方面是最有效的。为了扩大工艺规模，需要15-20%的高干物质（DM）浓度。然而，高DM水解和发酵受到材料的高粘度、较高的酶抑制和发酵微生物的限制。湿爆预处理方法使酶水解和同步糖化发酵（SSF）获得相对较高的产量时，未洗涤的浆料与14%DM和低酶负荷10 FPU/g纤维素在工业上可接受的时间范围为96小时。纤维素和半纤维素的酶水解转化率分别为70和68%，从SSF的总乙醇产率为68%。
Wheat straw was pretreated by wet explosion using three different oxidizing agents (H2O2, O-2, and air). The effect of the pretreatment was evaluated based on glucose and xylose liberated during enzymatic hydrolysis. The results showed that pretreatment with the use of O-2 as oxidizing agent was the most efficient in enhancing overall convertibility of the raw material to sugars and minimizing generation of furfural as a by-product. For scale-up of the process, high dry matter (DM) concentrations of 15-20% will be necessary. However, high DM hydrolysis and fermentation are limited by high viscosity of the material, higher inhibition of the enzymes, and fermenting microorganism. The wet-explosion pretreatment method enabled relatively high yields from both enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) to be obtained when performed on unwashed slurry with 14% DM and a low enzyme loading of 10 FPU/g cellulose in an industrial acceptable time frame of 96 h. Cellulose and hemicellulose conversion from enzymatic hydrolysis were 70 and 68%, respectively, and an overall ethanol yield from SSF was 68%.