5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells
5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells
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DOI：
10.1002/smll.201602363
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发表时间：
2017-03-07
期刊：
影响因子：
13.3
通讯作者：
Choi, In-Hong
中科院分区：
文献类型：
--
作者：
Kang, HanGoo;Kim, Seungjae;Choi, In-Hong
通过在小鼠模型中研究肥大细胞的活化和特应性皮炎的临床特征，评价银纳米颗粒（NPs）对过敏反应诱导的触发作用。颗粒释放在RBL-2 H3肥大细胞中由5 nm而非100 nm银NP诱导。5 nm银纳米颗粒诱导肥大细胞中活性氧（过氧化氢和线粒体超氧化物）和细胞内Ca++水平的增加。在由螨变应原诱导的特应性皮炎的小鼠模型中，与单独用变应原或用100 nm银NP和变应原处理的小鼠相比，在用5 nm银NP和变应原同时处理的小鼠中皮肤损伤更严重并且出现更早。组织学结果显示，在用5 nm银纳米颗粒和过敏原处理的小鼠中，类胰蛋白酶阳性肥大细胞的数量和血清中总IgE水平增加。本研究的结果表明，与5 nm银纳米粒子的共同治疗刺激肥大细胞脱颗粒，并诱导过敏倾向个体更早和更严重的临床变化。
The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca++ in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.