Isoelectric focusing in a drop.
Isoelectric focusing in a drop.
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等电聚焦在一滴中。
DOI：
10.1021/la104085t
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ansari,RafatR
中科院分区：
文献类型：
--
作者：
Weiss,NoahG;Hayes,MarkA;Garcia,AntonioA;Ansari,RafatR
研究了一种新的分子分离方法，称为液滴等电聚焦技术。液滴在超疏水表面上离散操纵，受到低电压等电聚焦，并分离，从而实现制备性分离。一种通用指示剂染料在两性离子缓冲液中产生稳定、可逆的pH梯度(3−10)，这些梯度导致蛋白质在液滴长度内聚焦。通过非侵入性光散射测量对聚焦进行视觉表征、光谱验证和定量评估。发现它与基于一维稳态理论的定量模型相关联。这项工作表明，分子分离可以部署在一个开放的液滴中，而不同的馏分可以分离成新的离散液体元素。
A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and splitresulting in a preparative separation. A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3−10) in ampholyte buffers, and these gradients lead to protein focusing within the drop length. Focusing was visually characterized, spectroscopically verified, and assessed quantitatively by noninvasive light scattering measurements. It was found to correlate with a quantitative model based on 1D steady-state theory. This work illustrates that molecular separations can be deployed within a single open drop, and the differential fractions can be separated into new discrete liquid elements.