Distribution and Strain Diversity of Immunoregulating Segmented Filamentous Bacteria in Human Intestinal Lavage Samples
Distribution and Strain Diversity of Immunoregulating Segmented Filamentous Bacteria in Human Intestinal Lavage Samples
复制标题
人肠灌洗样本中免疫调节分段丝状菌的分布和菌株多样性
DOI：
10.1007/s00248-019-01441-4
复制
发表时间：
2020-05-01
影响因子：
3.6
通讯作者：
Yin, Yeshi
中科院分区：
文献类型：
--
作者：
Chen, Huahai;Wang, Ling;Yin, Yeshi
分节丝状细菌（SF B）在宿主免疫调节中的功能是众所周知的，包括促进Th17细胞分化、B细胞成熟和免疫系统发育。然而，大多数SFB的分析都集中在动物模型上，因此，需要调查SFB在人类中的患病率及其在人类免疫调节和健康中的作用。尽管对人类中SFB的总体患病率知之甚少，但它们在断奶期间的动物中具有丰富的特征。在这项研究中，通过扫描电子显微镜（SEM）分析，在1至17岁的人类儿童的回肠灌洗液样本中检测到SFB样细菌，并观察它们插入粘膜。此外，通过免疫组织化学（IHC）和western blot观察SFB鞭毛蛋白在人细菌界面的表达。此外，两对SFB特异性引物，但针对不同的基因，用于检测SFB在人类肠道灌洗液样品。这些分析表明，SFB存在于超过50%的患者回肠样本中，与年龄无关。高通量基因测序结果表明，不同的SFB菌株之间的样品检测。9和23之间的SFB鞭毛蛋白基因的操作分类单位进行了鉴定。除了评估人类样本中SFB的患病率外，还评估了SFB存在与临床症状的主诉之间的相关性，以及SFB与患者血清免疫球蛋白浓度之间的关系。便血组SFB患病率（68%）明显高于腹痛组（56.10%）和腹泻组（43.75%）。此外，血清伊加，IgM和IgE的浓度，SFB阳性和SFB阴性患者组之间是相似的，虽然IgG浓度显着较高的SFB阴性组。
Segmented filamentous bacteria (SFB) are well known for their functions in the immunoregulation of hosts including the promotion of Th17 cell differentiation, B cell maturation, and immune system development. However, most analyses of SFB have focused on animal models, and thus, investigation of SFB prevalence in humans and their roles in human immunoregulation and health is needed. Although little is known overall of SFB prevalence in humans, they are characteristically abundant in animals during weaning. In this study, SFB-like bacteria were detected in ileal lavage samples from human children that were aged between 1 to 17 years old by scanning electron microscopy (SEM) analysis, and their insertion into the mucosa was also observed. In addition, the expression of SFB flagellin at the human bacterial interface was observed by immunohistochemistry (IHC) and western blot. Moreover, two pairs of primers specific for SFB, but targeting different genes, were used to detect SFB in human intestinal lavage samples. These analyses indicated that SFB were present in over 50% of patient ileal samples independent of age. High-throughput gene sequencing indicated that different SFB strains were detected among samples. Between nine and 23 SFB flagellin gene operational taxonomic units were identified. In addition to evaluating the prevalence of SFB in human samples, correlations between SFB presence and chief complaints of clinical symptoms were evaluated, as well as the relationship between SFB and patient serum immunoglobulin concentrations. SFB prevalence was significantly higher in hematochezia patients (68%) than in abdominal pain (56.10%) and diarrhea (43.75%) patients. Furthermore, the concentrations of serum IgA, IgM, and IgE, were similar between SFB-positive and SFB-negative patient groups, although IgG concentrations were significantly higher in the SFB-negative group.