CD8+ alphabeta T cells can mediate late airway responses and airway eosinophilia in rats.
CD8+ alphabeta T cells can mediate late airway responses and airway eosinophilia in rats.
复制标题
CD8 Alphata T 细胞可以介导大鼠的晚期气道反应和气道嗜酸性粒细胞增多。
DOI：
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发表时间：
2004
影响因子：
14.2
通讯作者：
James G. Martin
中科院分区：
文献类型：
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作者：
S. Isogai;R. Taha;M. Tamaoka;Y. Yoshizawa;Q. Hamid;James G. Martin
背景
CD 8 + T细胞亚群在介导迟发性过敏反应中的功能尚未完全了解。
目的
我们试图测试的假设，即CD 8 + α T细胞是促炎症的气道在体内通过使用一个良好的表征动物模型和过继转移技术。
方法
Brown Norway大鼠腹腔内给予从幼稚或卵清蛋白（OVA）致敏大鼠的淋巴结细胞中纯化的CD 8 + α T细胞（10 - 6），2天后用雾化的OVA进行攻击。对照大鼠皮下注射100 μ g溶于Al（OH）3的OVA致敏，或假注射盐水致敏，2周后进行OVA攻击。
结果
OVA致敏和OVA激发组以及OVA致敏的CD 8 + α-T细胞的受体与幼稚CD 8 + α-T细胞转移组和假致敏对照组相比，在激发后8小时内测量肺阻力，计算出显著的晚期气道反应。与初始CD 8 + α T细胞受体和假致敏对照组的数量相比，OVA致敏组和OVA致敏CD 8 + α T细胞受体中支气管肺泡灌洗液中嗜酸性粒细胞的数量增加。与假致敏组相比，OVA致敏组和OVA致敏CD 8 + α T细胞受体支气管肺泡灌洗液中IL-4和IL-5细胞因子mRNA表达增加。
结论
我们得出结论，抗原致敏的CD 8 + α T细胞可能有过敏原驱动的气道反应在大鼠的促炎作用。
BACKGROUND
The function of CD8+ T-cell subsets in mediating late allergic responses is incompletely understood.
OBJECTIVE
We sought to test the hypothesis that CD8+ alphabeta T cells are proinflammatory in the airways in vivo by using a well-characterized animal model and the technique of adoptive transfer.
METHODS
Brown Norway rats were administered CD8 + alphabeta T cells (10 6 ) intraperitoneally purified from lymph node cells of either naive or ovalbumin (OVA)-sensitized rats and were challenged with aerosolized OVA 2 days later. Control rats were sensitized to 100 mug of OVA in Al(OH) 3 subcutaneously or sham sensitized to saline and were OVA challenged 2 weeks later.
RESULTS
The OVA-sensitized and OVA-challenged group and the recipients of OVA-primed CD8+ alphabeta T cells had significant late airway responses calculated from lung resistance measured for an 8-hour period after challenge compared with the naive CD8 + alphabeta T cell-transferred group and the sham-sensitized control group. The number of eosinophils in bronchoalveolar lavage fluid increased in the OVA-sensitized group and the OVA-primed CD8+ alphabeta T-cell recipients compared with numbers in the naive CD8+ alphabeta T-cell recipients and the sham-sensitized control group. IL-4 and IL-5 cytokine mRNA expression in bronchoalveolar lavage fluid increased in the OVA-sensitized group and the OVA-primed CD8+ alphabeta T-cell recipients compared with that in the sham-sensitized group.
CONCLUSION
We conclude that antigen-primed CD8 + alphabeta T cells might have a proinflammatory role in allergen-driven airway responses in the rat.