The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2
The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2
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DOI：
10.1101/gad.13.22.2928
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发表时间：
1999-11-15
影响因子：
10.5
通讯作者：
Conaway, JW
中科院分区：
文献类型：
--
作者：
Kamura, T;Conrad, MN;Conaway, JW
环H2指蛋白Rbx1是相关的SCF(S位于bar KP1-(C)bar dc53/cul1-(F)bar dc53/cul1-(F)bar-box蛋白下)和von Hippel-Lindau(VHL)肿瘤抑制因子(细长蛋白BC-cul2-vhl)E3泛素连接酶复合体的一个亚基，它是CDC53/Rbx1和cul2/Rbx1模块的组成部分，通过E2泛素结合酶CDC34和Ubc5激活目标蛋白的泛素化。在这里，我们证明了CDC53/Rbx1和Cul2/Rbx1模块也通过专门的E2 Rub1结合酶Ubc12激活了泛素样蛋白Rub1与CDC53和Cul2的结合。我们的发现确定Rbx1是负责目标蛋白的泛素和Rub1修饰的酶系统的共同组成部分。
The RING-H2 finger protein Rbx1 is a subunit of the related SCF ((S) under bar kp1-(C) under bar dc53/Cul1-(F) under bar-box protein) and von Hippel-Lindau (VHL) tumor suppressor (elongin BC-Cul2-VHL) E3 ubiquitin ligase complexes, where it functions as a component of Cdc53/Rbx1 and Cul2/ Rbx1 modules that activate ubiquitination of target proteins by the E2 ubiquitin-conjugating enzymes Cdc34 and Ubc5. Here we demonstrate that the Cdc53/Rbx1 and Cul2/Rbx1 modules also activate conjugation of the ubiquitin-like protein Rub1 to Cdc53 and Cul2 by the dedicated E2 Rub1 conjugating enzyme Ubc12. Our findings identify Rbx1 as a common component of enzyme systems responsible for ubiquitin and Rub1 modification of target proteins.