Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array
Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array
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DOI：
10.1117/1.2976141
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发表时间：
2008-09-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Song, Liang;Maslov, Konstantin;Wang, Lihong V.
我们提出了一种体内暗场反射模式光声显微镜系统，该系统在50 Hz下进行实时波束形成的横断面（b扫描）成像，并在1 Hz下进行后波束形成的166个b扫描帧的三维成像。据我们所知，这是目前光声成像最快的速度。定制的光传输系统集成了一个30 mhz的超声线性阵列，实现了暗场反射模式成像。阵列的线性机械扫描产生三维图像。该系统的轴向、横向和仰角分辨率分别为25、70和200 μ m，可以对3 mm深的散射生物组织进行成像。大鼠皮下血管的体积图像在体内得到证实。快速三维光声显微镜有望促进光声成像在生物医学研究中的应用，这些研究涉及需要即时诊断的动力学和临床程序。(C) 2008光学仪器工程师学会。(DOI: 10.1117/1.2976141)
We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50 Hz with real-time beamforming and 3-D imaging consisting of 166 B-scan frames at 1 Hz with postbeamforming. To our knowledge, this speed is currently the fastest in photoacoustic imaging. A custom-designed light delivery system is integrated with a 30-MHz ultrasound linear array to realize dark-field reflection-mode imaging. Linear mechanical scanning of the array produces 3-D images. The system has axial, lateral, and elevational resolutions of 25, 70, and 200 mu m, respectively, and can image 3 mm deep in scattering biological tissues. Volumetric images of subcutaneous vasculature in rats are demonstrated in vivo. Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis. (C) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.2976141]