Assessment of accuracy of the structure-factor-size-estimator method in determining red blood cell aggregate size from ultrasound spectral backscatter coefficient.
Assessment of accuracy of the structure-factor-size-estimator method in determining red blood cell aggregate size from ultrasound spectral backscatter coefficient.
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DOI：
10.1121/1.3561653
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
R. Saha;E. Franceschini;G. Cloutier
中科院分区：
文献类型：
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作者：
R. Saha;E. Franceschini;G. Cloutier
讨论了利用计算机模拟红细胞团簇产生超声背向散射系数的方法。该模拟算法适用于生成非重叠、各向同性和相当相同的RBC簇。RBC按照六方密堆积（HCP）结构堆叠以形成致密的球形聚集体。重复这样的聚集体，并在三维空间中在非重叠条件下随机放置，以模拟聚集的血液样品。在750 KHz和200 MHz之间计算不同血细胞比容的各种簇大小的样品的BSC。在低频率（<20 MHz）下，BSC的幅度随着平均聚集体尺寸的增加而增加。结构因子尺寸估计（SFSE）方法在确定平均骨料尺寸和填充因子的准确性也进行了检查。对于每种红细胞压积，SFSE预测的聚集体平均大小与真实大小之间存在良好的相关性（R（2）≥ 0.94）。这项研究表明，对于球形聚集体，每个红细胞压积都存在一个SFSE工作最准确的区域。典型地，在40%血细胞比容下，对于14和17 μm之间的尺寸，SFSE估计平均簇大小的误差<20%。本研究表明，SFSE的理论框架下，各向同性骨料的假设是有效的。
A computer simulation study to produce ultrasonic backscatter coefficients (BSCs) from red blood cell (RBC) clusters is discussed. The simulation algorithm is suitable for generating non-overlapping, isotropic, and fairly identical RBC clusters. RBCs were stacked following the hexagonal close packing (HCP) structure to form a compact spherical aggregate. Such an aggregate was repeated and placed randomly under non-overlapping condition in the three-dimensional space to mimic an aggregated blood sample. BSCs were computed between 750 KHz and 200 MHz for samples of various cluster sizes at different hematocrits. Magnitudes of BSCs increased with mean aggregate sizes at low frequencies (<20 MHz). The accuracy of the structure-factor-size-estimator (SFSE) method in determining mean aggregate size and packing factor was also examined. A good correlation (R(2) ≥ 0.94) between the mean size of aggregates predicted by the SFSE and true size was found for each hematocrit. This study shows that for spherical aggregates there exists a region for each hematocrit where SFSE works most accurately. Typically, error of SFSE in estimating mean cluster size was <20% for dimensions between 14 and 17 μm at 40% hematocrit. This study suggests that the theoretical framework of SFSE is valid under the assumption of isotropic aggregates.